A novel two-chain IGF-II-derived peptide from purified β-cell granules

Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
Christina M BuchananGarth J S Cooper

Abstract

Insulin-like growth factor II (IGF-II) is a potent mitogen that regulates prenatal growth and development in both humans and rodents. Its role in post-natal life is less clear although immunohistochemical studies have observed IGF-II-like immunoreactivity (IGF-II-LI) associated with insulin-producing pancreatic β-cells. Here we isolated secretory granules from a β-cell line, βTC6-F7, and characterized the nature of the IGF-II-LI located therein. Secretory granules were isolated from cultured mouse βTC6-F7 cells by ultracentrifugation. Granule protein content was separated by reversed-phase HPLC, and assayed for IGF-II (radioimmunoassay) prior to identification by gas-phase NH(2)-terminal sequencing and MALDI-TOF MS. Effects of glucose incorporation into muscle glycogen were determined by incubating with isolated rat soleus muscle strips. βTC6-F7 cells contained 60 ± 8 pmol of IGF-II-LI per 10⁶ cells compared to 340 ± 44 pmol insulin-LI per 10⁶ cells. IGF-II immunoreactive fractions were found to contain an IGF-II-like molecule with a molecular mass of 6847.6 Da. The protein was found to be a two-chain insulin-like product of Igf2 that corresponds to mouse des(37-40)IGF-II, which we termed 'vesiculin'. This molecule was also det...Continue Reading

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Citations

Dec 6, 2011·Growth Factors·Lynda K Harris, Melissa Westwood
Apr 5, 2014·The Journal of Allergy and Clinical Immunology·Gui YangPing-Chang Yang
Mar 30, 2013·Organic & Biomolecular Chemistry·Geoffrey M WilliamsMargaret A Brimble

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