PMID: 12110919Jul 12, 2002Paper

A Novel Type of Apoptosis Induced by Cadmium in BA/F3beta Cells

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
Q ZhaiXin-Yuan Liu

Abstract

Apoptosis is usually accompanied by DNA fragmentation and up-regulation of reactive oxygen species, and it can be inhibited by overexpression of Bcl-2. Here, cadmium was found to induce apoptosis in BA/F3beta cells. MTT assay, Hochest 33258 staining, and transmission electron microscopy analysis were used to detect the apoptosis, however, neither DNA fragmentation nor up-regulation of reactive oxygen species were observed in this type of apoptosis. Furthermore, Bcl-2 overexpression had no effect on this type of apoptosis. In conclusion, these data suggested that cadmium induced a novel type of apoptosis in BA/F3beta cells.

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