PMID: 6406517Jun 1, 1983Paper

A novel type of cytoplasmic granule in bovine neutrophils

The Journal of Cell Biology
R GennaroM Baggiolini

Abstract

We obtained cell preparations containing greater than 95% neutrophils from freshly drawn bovine blood. The cells were suspended in sucrose and disrupted in a Dounce homogenizer, and the postnuclear supernate was fractionated by zonal differential sedimentation and by isopycnic equilibration. The subcellular fractions were characterized biochemically by testing for marker enzymes and other constituents known to occur in azurophil and specific granules of other species, and by electrophoretic analysis of extracts of the particulate material. In addition, each fraction was examined by random-sampling electron microscopy. We found that bovine neutrophils contain in addition to azurophil and specific granules a third type of granule, not known to occur in neutrophils of other species. These novel granules are larger, denser, and considerably more numerous than the two other types. Except for lactoferrin, they lack the characteristic constituents of azurophil granules (peroxidase, acid hydrolases, and neutral proteinases) and of specific granules (vitamin B12-binding protein). Instead, they contain a group of highly cationic proteins not found in the other granules, and they are the exclusive stores of powerful oxygen-independent bac...Continue Reading

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Citations

Sep 15, 1984·Experientia·M Baggiolini
Apr 1, 1993·Comparative Immunology, Microbiology and Infectious Diseases·S Sarmah, T More
Oct 1, 1985·Veterinary Immunology and Immunopathology·N Craven, M R Williams
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Nov 7, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S D SwainM T Quinn
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Jul 8, 2020·International Journal of Molecular Sciences·Leonie FingerhutNicole de Buhr
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Jan 1, 2014·Journal of Veterinary Medicine·Mohamed Ezzat AlnakipJorge Barros-Velázquez
Feb 16, 2018·Cell and Tissue Research·Laura L Bassel, Jeff L Caswell

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