A novel type of structurally simple nonpeptide inhibitors for alpha-chymotrypsin. Induced-fit binding of methyl 2-allyl-3-benzenepropanoate to the S2 subsite pocket

Bioorganic & Medicinal Chemistry
D H KimS J Chung

Abstract

Unexpectedly, methyl and benzyl esters of 2-allyl-3-benzenepropanoic acid were found to be not substrates but potent competitive inhibitors for alpha-chymotrypsin. The inhibitory property of the structurally simple nonpeptidic compounds is ascribed to their high binding affinity to the enzyme at the S2 rather than S1 subsite pocket. These inhibitors exist in a flexible form in solution, but as they bind to the enzyme bulky contrained conformers present in a minute concentration play an important role, forming tighter enzyme.inhibitor complexes by binding to the large hydrophobic S2 pocket. The contrained conformers are thought to be resulted from intramolecular CH/pi interactions between a vinylic proton and the aromatic pi-electron cloud in the inhibitor molecules. These compounds constitute novel examples of the induced-fit binding inhibitor of possibly simplest structure.

References

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Feb 1, 1958·Proceedings of the National Academy of Sciences of the United States of America·D E Koshland

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Citations

May 20, 2014·Physical Chemistry Chemical Physics : PCCP·Motohiro NishioPinak Chakrabarti
Mar 17, 2007·The American Journal of Bioethics : AJOB·Timothy Caulfield, Tania Bubela
Oct 26, 1999·Bioorganic & Medicinal Chemistry·Y UmezawaM Nishio
Jan 31, 2004·The Journal of Organic Chemistry·Panee C HutchisonDavid J Procter

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