A Novel UDP-Glycosyltransferase of Rhodiola crenulata Converts Tyrosol to Specifically Produce Icariside D2

BioMed Research International
Zhihua LiaoMin Chen

Abstract

Rhodiola crenulata is a Tibetan native herbal plant belonging to the family of Crassulaceae, which produces the pharmaceutical icariside D2 with the activities of inhibiting angiotensin-converting enzyme and killing leukemia cancer cells. In this study, we functionally characterized a novel UDP-glycosyltransferase (RcUGT1) that converted tyrosol to specifically produce icariside D2 from R. crenulata at molecular and biochemical levels. RcUGT1 was highly expressed in flowers and roots, while the icariside D2 content was much higher in stems than that in other organs, suggesting the potential translocation of icariside D2 from flowers and roots to stems. The high production of icariside D2 in stems provided a reasonable suggestion to farmers to harvest stems instead of roots for icariside D2 production. Enzymatic assays of recombinant RcUGT1 indicated that it converted tyrosol to specifically form icariside D2, with the values of Km 0.97±0.10 mM, Vmax 286±8.26 pKat/mg, Kcat 0.01552 s-1, and Kcat/Km 159.55 s-1 M-1. Functional identification of RcUGT1 facilitated the icariside D2 production through metabolic engineering in plants or synthetic biology in microbes.

References

Jul 1, 1987·Molecular Biology and Evolution·N Saitou, M Nei
Nov 2, 2011·International Journal of Food Sciences and Nutrition·Shun-Wan Chan
Jan 10, 2012·Journal of Agricultural and Food Chemistry·Ya-nan YangPei-cheng Zhang
Jul 21, 2014·Food Chemistry·Arunee SimaratanamongkolPharkphoom Panichayupakaranant
Oct 18, 2014·Scientific Reports·Yanfen BaiYanhe Ma
Apr 10, 2015·Pharmaceutical Biology·Nguyen Thi Thu HienYoung Ho Kim
Jan 26, 2016·Metabolic Engineering·Yanfen BaiYanhe Ma

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Citations

Jul 25, 2019·Frontiers in Bioengineering and Biotechnology·Wei GuoXu Fang
Jul 1, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fangfang FanXiaobo Wang

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Datasets Mentioned

BETA
MH299424

Methods Mentioned

BETA
PCR
enzymatic assay
glycosylation

Software Mentioned

MEGA
Vector NTI

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