A novel ultra high-performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of xanthones and steroidal saponins in crude and salt-processed Anemarrhenae Rhizoma aqueous extracts

Journal of Separation Science
De JiTulin Lu

Abstract

We established a rapid and sensitive ultra high-performance liquid chromatography tandem mass spectrometry method for the simultaneous quantification of xanthones and steroidal saponins in rat plasma. Chromatographic separation was achieved on a C18 column with a mobile phase comprising acetonitrile and 0.1% formic acid. The detection was performed by negative electrospray ionization in multiple reaction monitoring mode. The validated method showed good linearity within the tested range (r > 0.9945). The intra- and interday precision at high, medium, and low concentrations was less than 7.96%. The bias of accuracies ranged from -1.92 to 9.62%. The extraction recoveries of the compounds ranged from 84.78 to 88.69%, and the matrix effects ranged from 96.76 to 108.59%. This method was successfully applied to a pharmacokinetic comparison of crude and salt-processed Anemarrhenae Rhizoma aqueous extracts after oral administration in rats. The maximum plasma concentration and area under concentration-time curve of timosaponin BIII and timosaponin AIII increased significantly (P < 0.05 or 0.01) and those of timosaponin BII decreased significantly (P < 0.05) after processing. These results could contribute to the clinical application of...Continue Reading

References

Sep 29, 2006·Biomedical Chromatography : BMC·Hui WangCheng-Gang Huang
Mar 30, 2007·Journal of Chromatography. a·Geoffrey C KiteMonique S J Simmonds
Sep 29, 2009·Archives of Pharmacal Research·Wen-Quan LuWan-Sheng Chen
Apr 27, 2011·Clinical and Experimental Pharmacology & Physiology·Wen-Quan LuWan-Sheng Chen
Feb 26, 2013·International Journal of Molecular Sciences·Yanping LiuZhenzhen Cai
Feb 22, 2014·Journal of Ethnopharmacology·Yingli WangYanze Liu
Jul 13, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yi FengYiming Liu
Oct 23, 2014·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jingjing ShengGuoyu Pan
Aug 19, 2015·Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·Xia LeiHai-xue Kuang
May 1, 2012·Food Chemistry·Shaoying HouChanghao Sun
Mar 8, 2016·Journal of Pharmaceutical and Biomedical Analysis·Xiangjun QiuRuan-Min Zou
Jan 25, 2017·Korean Journal for Food Science of Animal Resources·Shaohua YangGuoqing Liu
Jul 1, 2017·Journal of Pharmaceutical and Biomedical Analysis·Si-Hui NianPing Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.

© 2022 Meta ULC. All rights reserved