PMID: 9443969Mar 14, 1998Paper

A novel UV-damaged DNA binding protein emerges during the chromatin-eliminating cleavage period in Ascaris suum

Nucleic Acids Research
C Seidl, K B Moritz

Abstract

During the early cleavage period of Ascaris suum , chromatin diminution takes place in the somatic founder cells. In the process of chromatin diminution numerous heterochromatic blocks, consisting predominantly of highly repeated DNA, are discarded during mitotic anaphase and are later on digested in the cytoplasm. Very little is known about proteins that are involved in chromatin diminution. We have detected a nuclear protein and purified it to near homogeneity by its preferential binding to UV-damaged DNA. We termed this protein chromatin diminution associated factor 1 (CDAF1), because maximum binding activity per nucleus was observed to develop in 4-8-cell stages, when chromatin diminution occurs for the first time. CDAF1 recognizes cyclobutane pyrimidine dimers in UV-damaged double-stranded DNA. Its binding properties identify CDAF1 as a novel kind of damaged-DNA binding protein. CDAF1 activity is almost not detectable in 1-celled embryos. It increases dramatically during formation of somatic founder cells and persists up to the first larval stage. However, CDAF1 is absent in tissues of adults. These findings led us to suggest that CDAF1 plays a dual role: during the early segregative cleavage period it might be involved in...Continue Reading

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Citations

Mar 25, 2000·International Journal for Parasitology·F Müller, H Tobler
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R Sanford, J P Bruzik
Jan 25, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Yi-Show LaiTodd Hsu
Jun 3, 2014·Current Opinion in Genetics & Development·Jianbin Wang, Richard E Davis

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