A novel vertebrate model of Staphylococcus aureus infection reveals phagocyte-dependent resistance of zebrafish to non-host specialized pathogens.

Cellular Microbiology
Tomasz K PrajsnarS A Renshaw

Abstract

With the emergence of multiply resistant Staphylococcus aureus, there is an urgent need to better understand the molecular determinants of S. aureus pathogenesis. A model of staphylococcal pathogenesis in zebrafish embryos has been established, in which host phagocytes are able to mount an effective immune response, preventing overwhelming infection from small inocula. Myeloid cell depletion, by pu.1 morpholino-modified antisense injection, removes this immune protection. Macrophages and neutrophils are both implicated in this immune response, phagocytosing circulating bacteria. In addition, in vivo phagocyte/bacteria interactions can be visualized within transparent embryos. A preliminary screen for bacterial pathogenesis determinants has shown that strains bearing mutations in perR, pheP and saeR are attenuated. perR and pheP mutants are deficient in growth in vivo, and their virulence is not fully restored by myeloid cell depletion. On the other hand, saeR mutants are able to grow in vivo, and are completely restored to virulence by myeloid cell depletion. Thus specific pathogen gene function can be matched with particular facets of host response. Zebrafish are a new addition to the tools available for the study of S. aureus...Continue Reading

References

Mar 1, 1978·Infection and Immunity·J Santoro, M E Levison
Jul 1, 1992·Infection and Immunity·C BunceN Barg
Nov 1, 1994·The Journal of Clinical Investigation·A L CheungA S Bayer
Jan 10, 1996·JAMA : the Journal of the American Medical Association·M J FineW N Kapoor
May 23, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G L Archer
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Dec 31, 1998·Antimicrobial Agents and Chemotherapy·J M EntenzaP Moreillon
Apr 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C E WillettA G Zapata
Aug 7, 1999·FEMS Microbiology Letters·A T GiraudoR Nagel
Oct 19, 2000·Arthritis and Rheumatism·M Verdrengh, A Tarkowski
Jul 4, 2001·Trends in Microbiology·A TarkowskiM Verdrengh
Sep 7, 2001·Annual Review of Microbiology·J K McCormickP M Schlievert
Mar 23, 2002·Current Biology : CB·Holger KnautChristiane Nüsslein-Volhard
Jun 18, 2002·Infection and Immunity·Melody N NeelyMichael Caparon
Jun 25, 2002·Microbial Pathogenesis·Chikara KaitoKazuhisa Sekimizu
Dec 14, 2002·FEMS Microbiology Letters·Andrej TarkowskiMargareta Verdrengh
Mar 26, 2003·Infection and Immunity·Costi D SifriStephen B Calderwood
May 7, 2003·Current Microbiology·Ana T GiraudoRosa Nagel
Aug 20, 2003·Cellular Microbiology·Astrid M van der SarWilbert Bitter
Oct 3, 2003·Microbiology·Michail H KaravolosSimon J Foster
Dec 23, 2003·Molecular Immunology·Cyril JaultJohanna Chluba
Dec 23, 2003·Molecular Immunology·Annemarie H MeijerHerman P Spaink
Jan 2, 2004·Microbiology·Lindsey ShawSimon J Foster
Feb 6, 2004·The Journal of Antimicrobial Chemotherapy·William J WeissLefa Alksne
Apr 16, 2004·Immunity·Nikolaus S TredeLeonard I Zon
Jun 15, 2004·Microbial Pathogenesis·Ronan O'TooleHans Wolf-Watz
Jun 29, 2004·Nature Immunology·Christine L CosmaLalita Ramakrishnan
Jul 17, 2004·Microbiology·Andrew J NeedhamSimon J Foster
Dec 4, 2004·Journal of Bacteriology·Bret M BentonJerry M Buysse
Dec 29, 2004·Developmental Cell·Jennifer RhodesJohn P Kanki
Feb 15, 2005·FEMS Immunology and Medical Microbiology·Jorge García-LaraSimon J Foster
Mar 9, 2005·Developmental and Comparative Immunology·Meagan E PressleyCarol H Kim
Oct 12, 2005·Archives of Medical Research·Gonzalo M L Bearman, Richard P Wenzel

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Citations

Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jorge Galindo-VillegasVictoriano Mulero
Feb 7, 2012·Antioxidants & Redox Signaling·Stephen Spiro, Benoît D'Autréaux
Jun 24, 2009·Infection and Immunity·Jean-Pierre LevraudMarc Lecuit
Oct 24, 2012·Infection and Immunity·Elizabeth A HarvieAnna Huttenlocher
Sep 5, 2013·Infection and Immunity·Tomasz K PrajsnarStéphane Mesnage
Jan 27, 2009·Infection and Immunity·Anne E ClatworthyDeborah T Hung
Jan 10, 2012·Disease Models & Mechanisms·Stephen A Renshaw, Nikolaus S Trede
Sep 26, 2013·International Journal of Molecular Sciences·Evelina PapaioannouWim J Quax
Aug 9, 2012·Journal of Visualized Experiments : JoVE·Kimberly M Brothers, Robert T Wheeler
Feb 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Audrey BernutLaurent Kremer
Jul 16, 2014·Zebrafish·Amorce LimaBurt Anderson
Jun 4, 2015·Emerging Microbes & Infections·Jun XiePatrick Cy Woo
Jan 19, 2016·Developmental and Comparative Immunology·John E McGonigleJens Rolff
Oct 2, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Ya-juan Li, Bing Hu
Feb 16, 2010·Current Opinion in Immunology·Michelle Kanther, John F Rawls
Mar 9, 2010·Microbial Pathogenesis·Zongfu WuChengping Lu
Jan 5, 2010·Current Opinion in Microbiology·David O'Callaghan, Annette Vergunst
Feb 1, 2012·Diabetes/metabolism Research and Reviews·Jean-Louis RichardAlbert Sotto
Jul 31, 2012·Cell Reports·Kevin TakakiLalita Ramakrishnan
Jan 21, 2016·International Journal of Mycobacteriology·Jonnalagadda Padma SrideviDharmarajan Sriram
Oct 1, 2013·Developmental and Comparative Immunology·Anni SaralahtiSamuli Rounioja
Feb 24, 2015·Frontiers in Microbiology·Yamilé López HernándezIsidre Gibert
Dec 31, 2014·Zebrafish·Usua OyarbideMiguel Angel Pardo
Jul 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Iman HalloumLaurent Kremer

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