A novel video tracking method to evaluate the effect of influenza infection and antiviral treatment on ferret activity

PloS One
Ding Yuan OhAeron C Hurt

Abstract

Ferrets are the preferred animal model to assess influenza virus infection, virulence and transmission as they display similar clinical symptoms and pathogenesis to those of humans. Measures of disease severity in the ferret include weight loss, temperature rise, sneezing, viral shedding and reduced activity. To date, the only available method for activity measurement has been the assignment of an arbitrary score by a 'blind' observer based on pre-defined responsiveness scale. This manual scoring method is subjective and can be prone to bias. In this study, we described a novel video-tracking methodology for determining activity changes in a ferret model of influenza infection. This method eliminates the various limitations of manual scoring, which include the need for a sole 'blind' observer and the requirement to recognise the 'normal' activity of ferrets in order to assign relative activity scores. In ferrets infected with an A(H1N1)pdm09 virus, video-tracking was more sensitive than manual scoring in detecting ferret activity changes. Using this video-tracking method, oseltamivir treatment was found to ameliorate the effect of influenza infection on activity in ferret. Oseltamivir treatment of animals was associated with an...Continue Reading

References

Apr 1, 1989·Journal of Virological Methods·P D ReumanG M Schiff
Jan 1, 1981·Journal of Clinical Microbiology·A S Monto, H F Maassab
Oct 16, 1999·Lancet·N J Cox, K Subbarao
Jun 23, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Saikat BoliarThomas M Chambers
Feb 14, 2007·Antimicrobial Agents and Chemotherapy·Elena A GovorkovaRobert G Webster
Jun 20, 2007·American Journal of Infection Control·Ji-Ming ChenYing-Xue Sun
Sep 4, 2007·American Journal of Infection Control·Ji-Ming ChenYing-Xue Sun
Oct 9, 2007·The Journal of Infectious Diseases·Miriam E R DarnellDeborah R Taylor
Apr 22, 2008·The Journal of Infectious Diseases·David A BoltzElena A Govorkova
Apr 17, 2012·Physiology & Behavior·Charles C HornBrian A Williams
Dec 15, 2012·Scientific Reports·James P LongJohn E Bigger
Mar 9, 2013·PloS One·Gregory V StarkEric M Vela
May 15, 2013·PLoS Pathogens·Teagan GuarnacciaKaren L Laurie
Jul 5, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Florian Duclot, Mohamed Kabbaj
Jan 16, 2014·ELife·Inbal Ben-Ami BartalPeggy Mason

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Citations

Feb 13, 2016·Frontiers in Microbiology·Ding Y Oh, Aeron C Hurt
May 19, 2016·Emerging Infectious Diseases·Aeron C Hurt, Heath Kelly
Jul 15, 2016·Microbiology and Molecular Biology Reviews : MMBR·Jessica A BelserTaronna R Maines
Oct 27, 2018·Expert Opinion on Drug Discovery·Edin J MifsudAeron C Hurt
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·David J BealeAeron C Hurt

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