A novel WRKY transcriptional factor from Thlaspi caerulescens negatively regulates the osmotic stress tolerance of transgenic tobacco

Plant Cell Reports
Wei WeiTuanyao Chai

Abstract

A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY53 contains a 975-bp open reading frame encoding a putative protein of 324 amino acids. Homology searches showed that TcWRKY53 resembles similar WRKY domain-containing proteins from rice, parsley and tobacco, especially AtWRKY53 from Arabidopsis thaliana. Semi-quantitative RT-PCR showed that the expression of TcWRKY53 was strongly induced by various environmental stresses, including an excess of NaCl, drought, cold and the signal molecule salicylic acid (SA). The expression of TcWRKY53 in response to NaCl, drought and cold suggested a possible role of TcWRKY53 in abiotic stress response. However, physiological tests indicated that the expression of TcWRKY53 in tobaccos decreases tolerance to sorbitol during seedling root development. This was consistent with PEG6000 treatment of tobacco seedlings, and together these results indicate a negative modulation of TcWRKY53 in response to osmotic stress. Furthermore, two et...Continue Reading

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Citations

Aug 17, 2010·Yi chuan = Hereditas·Qian-Tang Fu, Di-Qiu Yu
Oct 4, 2011·Biochimica Et Biophysica Acta·Ligang ChenDiqiu Yu
Apr 15, 2008·Plant Physiology and Biochemistry : PPB·Markus Georg HofmannThomas Roitsch
Nov 20, 2008·Journal of Integrative Plant Biology·Giovanni DalCorsoAntonella Furini
Apr 21, 2010·Plant Signaling & Behavior·Giovanni DalCorsoAntonella Furini
Apr 17, 2015·TheScientificWorldJournal·Aditya Banerjee, Aryadeep Roychoudhury
Oct 9, 2009·Molecular Plant-microbe Interactions : MPMI·Haiyan JiaGary J Muehlbauer
Apr 17, 2008·Plant & Cell Physiology·Rengasamy RamamoorthySrinivasan Ramachandran
Jan 9, 2019·Plant Molecular Biology·Yangyang HanShuqing Cao
Feb 21, 2018·Frontiers in Plant Science·Siddhi K JalmiAlok K Sinha

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