A Novel Zebrafish (Danio rerio) Assay for Assessing Musk Ambrette-Induced Toxicity

Bulletin of Environmental Contamination and Toxicology
Li QuWeimin Song

Abstract

Musk ambrette (4-tert-butyl-3-methoxy-2,6-dinitrotoluene) is a nitro musk, a cheap substitute for natural musk and a potential environmental pollutant based on its persistence, accumulation in human organisms. We investigated the acute toxicity of musk ambrette using wild-type AB and transgenic Tg(fli1a:EGFP)y1 zebrafish. Different concentrations were delivered to zebrafish by direct soaking from 6 to 72 h post-fertilization (hpf). The LC50 of musk ambrette was 76.4 µg mL-1. As musk ambrette concentration increased, zebrafish embryos showed developmental delays (50 µg mL-1, 22 hpf), pericardial edema (5 µg mL-1, 48 hpf), circulatory disturbances, curved body axis (1 µg mL-1, 72 hpf) and death (100 µg mL-1, 22 hpf). Target organ toxicity was evaluated by a zebrafish angiogenesis model. Musk ambrette induced cardiovascular morphological changes, vessel permeability variation, angiogenic changes and cardiotoxicity (10 µg mL-1, 48 hpf). The disappearance of caudal vein plexus confirmed the vascular development toxicity. Musk ambrette negatively affects early life-stage survival and demonstrates various toxicities in zebrafish.

References

Aug 1, 1979·The Journal of Investigative Dermatology·I E KocheverL C Harber
Sep 30, 1984·Toxicology and Applied Pharmacology·P S SpencerR A Ford
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Jun 28, 2002·Cancer Cell·Joanne ChanThomas M Roberts
Aug 9, 2002·Developmental Biology·Nathan D Lawson, Brant M Weinstein
Mar 18, 2004·Archives of Environmental Contamination and Toxicology·G Carlsson, L Norrgren
Feb 13, 2008·Chemosphere·W M ShekPaul K S Lam
Jun 18, 2010·CA: a Cancer Journal for Clinicians·Kristina M Cook, William D Figg
Feb 12, 2011·Bulletin of Environmental Contamination and Toxicology·Zhengjun HuYaqi Cai
Mar 13, 2014·Environmental Health : a Global Access Science Source·Kathryn M TaylorJames Shine

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