A novel zebrafish human tumor xenograft model validated for anti-cancer drug screening

Molecular BioSystems
Da-Woon JungDarren R Williams

Abstract

The development of a relatively simple, reliant and cost-effective animal test will greatly facilitate drug development. In this study, our goal was the establishment of a rapid, simple, sensitive and reproducible zebrafish xenograft model for anti-cancer drug screening. We optimized the conditions for the cancer cell xenograft in terms of injected cell numbers, incubation temperature and time. A range of human carcinoma cell types were stained with a fluorescent dye prior to injection into the fish larvae. Subsequent cancer cell dissemination was observed under fluorescent microscopy. Differences in injected cell numbers were reflected in the rate of dissemination from the xenograft site. Paclitaxel, known as a microtubule stabilizer, dose-dependently inhibited cancer cell dissemination in our zebrafish xenograft model. An anti-migratory drug, LY294002 (phosphatidylinositol 3-kinase inhibitor) also decreased the cancer cell dissemination. Chemical modifications to increase cancer drug pharmacokinetics, such as increased solubility (17-DMAG compared to geldanamycin) could also be assessed in our xenograft model. In addition to testing our new model using known anti-cancer drugs, we carried out further validation by screening a ...Continue Reading

References

Feb 22, 1979·Nature·P B SchiffS B Horwitz
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·L WhitesellL M Neckers
Oct 24, 1998·Bioorganic & Medicinal Chemistry·S L Schreiber
May 17, 2003·Nature Reviews. Drug Discovery·Konrad H BleicherAlexander I Alanine
Aug 2, 2003·Nature Biotechnology·Franz B PichlerDonald R Love
Sep 25, 2003·Journal of the American Chemical Society·Sonya M KhersonskyYoung-Tae Chang
Oct 4, 2003·Nature Reviews. Drug Discovery·Mark A Lindsay
Apr 21, 2004·Nature Biotechnology·Randall T PetersonMark C Fishman
Jun 22, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lisa M J LeeMary J C Hendrix
Sep 3, 2005·Chemical Society Reviews·David R Spring
Jul 25, 2006·Advances in Cancer Research·Swee Sharp, Paul Workman
Apr 6, 2007·Cancer Research·Stefania NicoliMarco Presta
Apr 19, 2007·Nature Reviews. Genetics·Graham J Lieschke, Peter D Currie
Apr 26, 2007·ACS Chemical Biology·Laurent Ségalat
Aug 7, 2007·Orphanet Journal of Rare Diseases·Laurnet Ségalat
Apr 30, 2009·BMC Cancer·Ines J MarquesChristoph P Bagowski
Apr 1, 2010·Disease Models & Mechanisms·Marina C Mione, Nikolaus S Trede
Apr 23, 2010·Current Opinion in Chemical Biology·James R PorterKristopher M Depew
Apr 29, 2010·British Journal of Pharmacology·Jean Giacomotto, Laurent Ségalat
Jan 14, 2011·Haematologica·Benoist PruvotEric Solary
Jan 26, 2011·Annual Review of Pathology·Shu Liu, Steven D Leach
Feb 22, 2011·Trends in Cardiovascular Medicine·Karni S MoshalTinchung Leung
Mar 8, 2011·Experimental Cell Research·Lidia CerquettiAntonio Stigliano
Apr 27, 2011·British Journal of Haematology·Dale P CorkeryJason N Berman

❮ Previous
Next ❯

Citations

Jun 20, 2014·Nature Reviews. Cancer·Gloria M Slattum, Jody Rosenblatt
Mar 16, 2013·Journal of Fish Diseases·Z S BrowningM W Lenox
Nov 18, 2014·Biochemical Society Transactions·Da-Woon JungDarren R Williams
Oct 11, 2016·Computational and Structural Biotechnology Journal·Charles H Williams, Charles C Hong
Jun 26, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Miloš VittoriTamara Lah Turnšek
Apr 17, 2018·Médecine sciences : M/S·Pamela VölkelPierre-Olivier Angrand
Oct 17, 2017·Korean Circulation Journal·Gyojeong GuHae Young Lee
Jan 8, 2019·PloS One·Maria Lorena Cordero-MaldonadoJan de Sonneville
Jul 18, 2019·Journal of Experimental & Clinical Cancer Research : CR·Rossella ZaninGuidalberto Manfioletti
Oct 22, 2016·Oncotarget·Nicole S NicholasClaire M Wells
Jul 25, 2019·Journal of Translational Medicine·Ahmed Al-SamadiTuula Salo
Jan 1, 2017·Current Molecular Biology Reports·Sven KappelAnna Borgström
Jul 11, 2020·Cancer Gene Therapy·David MealieaJ Andrea McCart
Dec 20, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Brandon MolinaStephanie Grainger
Jun 3, 2021·Pharmaceuticals·Hung-Chieh LeeHuai-Jen Tsai
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Kelly Z MiaoHui Feng
Feb 22, 2014·ACS Applied Materials & Interfaces·Dickson JosephKurt E Geckeler
Aug 31, 2019·Bulletin du cancer·Ludivine RabyPierre-Olivier Angrand

❮ Previous
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