PMID: 6172535Dec 1, 1981Paper

A nuclear antigen associated with cell proliferation and blast transformation

The Journal of Experimental Medicine
Y TakasakiE M Tan

Abstract

A nuclear antigen associated with cell proliferation (proliferating cell nuclear antigen-PCNA) and blast transformation is recognized by autoantibodies in the sera of some patients with systemic lupus erythematosus. This autoantibody is a precipitating antibody and also reacts in immunofluorescence, staining the nucleoplasm of proliferating and blast-transformed cells. The autoantibody was used as a reagent to determine the distribution of PCNA in a synchronized continuous B lymphoid cell line (WiL-2) and in mitogen-induced blast-transformed lymphocytes. In WiL-2 cells, PCNA was detected as speckled nucleoplasmic staining in G1, S, and G2 phases of the cell cycle. In addition, during late G1 and early S phases, PCNA was also detected in the nucleolus. During mitogen-induced blast transformation of lymphocytes, PCNA was noticed in the nucleolus before the initiation of DNA synthesis and later became nucleoplasmic with disappearance of nucleolar staining. These studies demonstrate that the relationship of PCNA to proliferation and blast transformation may be associated with events related to DNA synthesis in these cells.

References

May 1, 1975·The Journal of Clinical Investigation·M A Alspaugh, E M Tan
Sep 1, 1968·Cancer·J A LevyV Defendi
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Y MoroiE M Tan

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Citations

Mar 1, 1996·The Journal of Pathology·T TakemuraK Yoshioka
Feb 1, 1995·Journal of Cellular Physiology·K TakaseE W Gelfand
Jan 1, 1992·Archives of Dermatological Research·F FurukawaD A Norris
Apr 1, 1997·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·J WatanabeL Zhou
Jan 1, 1995·Acta neurochirurgica·H KawamotoT Hirohata
Jul 1, 1989·Somatic Cell and Molecular Genetics·D H KuR Baserga
Sep 1, 1993·Clinical Rheumatology·Y TokanoS Hirose
Dec 17, 2009·Investigational New Drugs·Pandurangan Ashokkumar, Ganapasam Sudhandiran
Jul 1, 1995·Archives of Oral Biology·J T WrightJ W Spalding
Aug 1, 1990·Brain Research. Developmental Brain Research·K NegishiW K Stell
Aug 21, 1992·Brain Research. Developmental Brain Research·K NegishiT Saito
Jan 17, 1991·Biochimica Et Biophysica Acta·R A Bambara, C B Jessee
Sep 1, 1988·Molecular and Cellular Probes·S Whittingham, L J McNeilage
Feb 21, 2004·Medical Hypotheses·Alan N Elias
Aug 17, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A GemmaS Kudoh
Oct 20, 1998·Progress in Retinal and Eye Research·H J WagnerS P Collin
Nov 20, 1998·Neuropathology and Applied Neurobiology·C Bouvier-LabitD Figarella-Branger
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J E CelisA Celis
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R Bravo
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J E Celis, A Celis
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J M AlmendralR Bravo
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L JacobJ F Bach
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I SuzukaT Moriuchi
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D E CoolR L Margolis

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