PMID: 9182662Jun 16, 1997Paper

A nuclear-coded chloroplastic inner envelope membrane protein uses a soluble sorting intermediate upon import into the organelle

The Journal of Cell Biology
J LübeckJ Soll

Abstract

The chloroplastic inner envelope protein of 110 kD (IEP110) is part of the protein import machinery in the pea. Different hybrid proteins were constructed to assess the import and sorting pathway of IEP110. The IEP110 precursor (pIEP110) uses the general import pathway into chloroplasts, as shown by the mutual exchange of presequences with the precursor of the small subunit of ribulose-1,5-bisphosphate carboxylase (pSSU). Sorting information to the chloroplastic inner envelope is contained in an NH2-proximal part of mature IEP110 (110N). The NH2-terminus serves to anchor the protein into the membrane. Large COOH-terminal portions of this protein (80-90 kD) are exposed to the intermembrane space in situ. Successful sorting and integration of IEP110 and the derived constructs into the inner envelope are demonstrated by the inaccessability of processed mature protein to the protease thermolysin but accessibility to trypsin, i.e., the imported protein is exposed to the intermembrane space. A hybrid protein consisting of the transit sequence of SSU, the NH2-proximal part of mature IEP110, and mature SSU (tpSSU-110N-mSSU) is completely imported into the chloroplast stroma, from which it can be recovered as soluble, terminally process...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1992·Trends in Biochemical Sciences·B S GlickG Schatz
Aug 1, 1991·European Journal of Biochemistry·J JoyardR Douce
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S MarshallE Vierling
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M SalomonJ Soll
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Sep 1, 1995·The Journal of Biological Chemistry·S BrinkU I Flügge
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·P HugueneyB Camara
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J YuanK Cline
Jun 1, 1993·The Journal of Cell Biology·B R Miller, M G Cumsky
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H D BernsteinP Walter
Jul 1, 1996·Trends in Biochemical Sciences·R A Stuart, W Neupert
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·F Kessler, G Blobel
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Cline, R Henry

❮ Previous
Next ❯

Citations

Aug 16, 2008·Plant Molecular Biology·Ewa Firlej-KwokaBettina Bölter
Jan 25, 2005·Plant Biology·T BeckerE Schleiff
Mar 3, 2010·The Journal of Biological Chemistry·Antonio A B VianaDanny J Schnell
Sep 26, 2006·The Plant Cell·Shin-ya MiyagishimaKatherine W Osteryoung
Apr 24, 1999·The Plant Cell·K Keegstra, K Cline
Mar 3, 2010·Annual Review of Plant Biology·Hsou-min Li, Chi-Chou Chiu
Oct 4, 2011·BMC Plant Biology·Erika Kovács-BogdánBettina Bölter
Nov 15, 2000·Biological Chemistry·U C Vothknecht, J Soll
Oct 25, 2006·The Journal of Cell Biology·Ming Li, Danny J Schnell
May 18, 2007·FEBS Letters·Lea VojtaBettina Bölter
Apr 1, 2004·The Plant Journal : for Cell and Molecular Biology·Diane ConstanPaul Jarvis
Jun 11, 2003·The Plant Journal : for Cell and Molecular Biology·Jason L BurkheadMarinus Pilon
Jun 16, 2006·The Plant Journal : for Cell and Molecular Biology·Changcheng XuChristoph Benning
Dec 17, 2008·The New Phytologist·Paul Jarvis
Jul 29, 2008·The Plant Journal : for Cell and Molecular Biology·Chi-Chou Chiu, Hsou-Min Li
May 29, 2013·The Plant Journal : for Cell and Molecular Biology·Jia-Yin TsaiChwan-Deng Hsiao
Jun 23, 2011·Chembiochem : a European Journal of Chemical Biology·Bettina Bölter, Jürgen Soll
Jan 27, 2010·Biochimica Et Biophysica Acta·Erika Kovács-BogdánBettina Bölter
Dec 29, 2004·Current Biology : CB·Paul Jarvis, Colin Robinson
Oct 16, 2012·Biochimica Et Biophysica Acta·Lan-Xin Shi, Steven M Theg
Aug 5, 2010·Biochimica Et Biophysica Acta·Serena SchwenkertBettina Bölter
Oct 11, 2017·Journal of Experimental Botany·Rajneesh Singhal, Donna E Fernandez
Jun 8, 2004·The Journal of Biological Chemistry·Friederike HörmannJürgen Soll
Jul 23, 2003·The Journal of Biological Chemistry·Takehito InabaDanny J Schnell
Aug 24, 1999·The Journal of Biological Chemistry·A KouranovD J Schnell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.