A nuclear-encoded intein in the fungal pathogen Cryptococcus neoformans

Yeast
Margaret I ButlerRussell T M Poulter

Abstract

We have used comparative sequence analysis to identify an intein-like sequence (protein splicing element) present in Cryptococcus neoformans, a fungal pathogen of humans. The sequence encoding this element is present in the C. neoformans PRP8 gene, as an in-frame insertion relative to the PRP8 genes of other organisms. It contains sequences similar to those of the protein-splicing domains of two previously described yeast inteins (in Saccharomyces cerevisiae and Candida tropicalis), although it lacks any recognizable internal endonuclease domain. The Cryptococcus neoformans intein (Cne PRP8) is only the second to be found in a eukaryote nuclear genome; the previously described yeast inteins occur at the same site in the VMA gene homologues of S. cerevisiae and C. tropicalis. The host gene of the Cryptococcus intein, PRP8, encodes a highly conserved mRNA splicing protein found as part of the spliceosome. The Cne PRP8 intein may be a useful drug target in addressing the cryptococcal infections so prevalent in AIDS patients.

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Citations

Nov 8, 2011·BMC Evolutionary Biology·Adi BarzelLilach Hadany
Jun 2, 2006·BMC Evolutionary Biology·Margaret I ButlerRussell T M Poulter
Oct 31, 2006·BMC Biology·Timothy J D GoodwinRussell T M Poulter
Apr 21, 2005·RNA·Richard J Grainger, J D Beggs
Oct 26, 2016·Frontiers in Microbiology·José A L FernandesRaquel Cordeiro Theodoro
Mar 3, 2009·Journal of Basic Microbiology·Skander ElleucheStefanie Pöggeler
Apr 26, 2005·Yeast·Margaret I ButlerRussell T M Poulter
Aug 12, 2004·FEBS Letters·Xiang-Qin Liu, Jing Yang
Jun 24, 2009·Memórias do Instituto Oswaldo Cruz·Raquel Cordeiro Theodoro, Eduardo Bagagli
Jul 3, 2007·Biochimica Et Biophysica Acta·Esther J PearlSigurd M Wilbanks
Apr 6, 2005·Fungal Genetics and Biology : FG & B·Margaret I Butler, Russell T M Poulter
Dec 12, 2017·Medical Mycology·Hans Garcia GarcesEduardo Bagagli
Feb 9, 2018·Mobile DNA·Cathleen M GreenM Belfort
Mar 18, 2006·Applied Microbiology and Biotechnology·Skander ElleucheStefanie Pöggeler
Jun 22, 2019·Emerging Microbes & Infections·Zhong LiHongmin Li
Jan 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Zhong LiHongmin Li
Dec 20, 2020·Microorganisms·Ananya NandaSasmita Nayak

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