A Nuclear Export Block Triggers the Decay of Newly Synthesized Polyadenylated RNA.

Cell Reports
Agnieszka TudekTorben Heick Jensen

Abstract

Genomes are promiscuously transcribed, necessitating mechanisms that facilitate the sorting of RNA for function or destruction. The polyA (pA) tail is one such distinguishing feature, which in the Saccharomyces cerevisiae nucleus is bound by the Nab2p protein, yielding transcript protection. As Nab2p also contacts the main nuclear export factor Mex67p, we asked whether transport kinetics contributes to RNA sorting. Indeed, 3' end sequencing of newly transcribed pA+ RNAs demonstrates that nuclear depletion of Mex67p elicits their instant and global decay. A similar phenotype is evident upon inactivation of other export factors and proportional to the amount of nuclear pA+ RNA. As RNA expression is partially rescued by Nab2p overexpression, we propose that an export block out-titrates Nab2p onto nuclear-retained pA+ RNA, reducing the pool of Nab2p available to protect new transcripts. More generally, we suggest that nuclear RNA decay, negotiated by Nab2p availability, aids in balancing cellular transcript supply with demand.

Citations

Dec 24, 2018·Protein Science : a Publication of the Protein Society·Milo B FaskenMurray Stewart
Sep 23, 2020·Nucleic Acids Research·Yuhui DouTorben Heick Jensen
Dec 6, 2018·Current Genetics·Agnieszka TudekTorben Heick Jensen
Nov 3, 2020·Nucleic Acids Research·Lisbeth-Carolina AguilarBen Montpetit
May 5, 2021·Molecular and Cellular Biology·Irfana SaleemVimla Band
Aug 18, 2021·Nature Communications·Agnieszka TudekAndrzej Dziembowski
Aug 14, 2021·Genes & Development·Matti TurtolaTorben Heick Jensen
Jun 20, 2021·Molecular Microbiology·Amartya MishraLaurie K Read
Aug 21, 2021·Molecular Microbiology·Tom DendoovenBen F Luisi

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Datasets Mentioned

BETA
GSE108550
Raw
GSM2551210
GSM617027
GSE25107
GSM1706520
GSE57385
GSE108477

Methods Mentioned

BETA
immunoprecipitation
ChIP
X-ray
PCR
electrophoresis
RNaseq

Software Mentioned

QuantSeq
RNaseq
Lexogen
R package DESeq2
deepTools2
ENSEMBL
DESeq2
ArrayExpress
Genome Browser
ImageJ

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