A nucleostemin-like GTPase required for normal apical and floral meristem development in Arabidopsis.

Molecular Biology of the Cell
Xiaomin WangZonglie Hong

Abstract

Mammalian nucleostemin (NS) is preferentially expressed in stem cells and acts to promote cell cycle progression. In plants, stem cell activities have to be terminated during flower development, and this process requires the activation of AGAMOUS (AG) gene expression. Here, a nucleostemin-like 1 gene, NSN1, is shown to be required for flower development in Arabidopsis. The NSN1 mRNA was found in the inflorescence meristem and floral primordia, and its protein was localized to the nucleoli. Both heterozygous and homozygous plants developed defective flowers on inflorescences that were eventually terminated by the formation of carpelloid flowers. Overexpression of NSN1 resulted in loss of apical dominance and formation of defective flowers. Expression of the AG gene was found to be up-regulated in nsn1. The carpelloid flower defect of nsn1 was suppressed by the ag mutation in the nsn1 ag double mutant, whereas double mutants of nsn1 apetala2 (ap2) displayed enhanced defective floral phenotypes. These results suggest that in the delicately balanced regulatory network, NSN1 acts to repress AG and plays an additive role with AP2 in floral organ specification. As a midsize nucleolar GTPase, NSN1 represents a new class of regulatory p...Continue Reading

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Citations

Nov 8, 2011·Plant Molecular Biology·Xiaomin WangZonglie Hong
Oct 19, 2017·Plant Molecular Biology·Young JeonHyun-Sook Pai
Feb 2, 2019·BMC Plant Biology·Mona MazaheriShawn M Kaeppler
Oct 17, 2014·The Plant Journal : for Cell and Molecular Biology·Benjamin L WeisEnrico Schleiff

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Datasets Mentioned

BETA
AAV74413
AAH11720
AAO19472
EAL25205

Methods Mentioned

BETA
GTPase
PCR
transgenic
dissection
scanning electronic microscopy

Software Mentioned

ClustalX
ExPASy
Phylip
TreeView
Bootstrap

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