PMID: 11318106Apr 25, 2001Paper

A nucleus-encoded suppressor defines a new factor which can promote petD mRNA stability in the chloroplast of Chlamydomonas reinhardtii

Current Genetics
D EspositoJ Girard-Bascou

Abstract

Mutations in the Chlamydomonas reinhardtii nuclear gene MCD1 specifically destabilize the chloroplast petD mRNA, which encodes subunit IV of the cytochrome b6/f complex. The MCD1 gene product is thought to interact with the mRNA 5' end to protect it from degradation by a 5' --> 3' exoribonuclease and may also have a role in translation initiation. Here we report the isolation and characterization of a semidominant, allele-specific, nucleus-encoded suppressor of the mcd1-2 mutation. The suppressor mutation, which defines a new locus MCD2, allows accumulation of 10% of the wild-type level of petD mRNA and as much as 50% of the wild-type subunit IV level. Taken together, these results suggest the suppressor mutation restores photosynthetic growth by stabilizing petD mRNA. In addition, it may promote increased translational efficiency, an inference supported by direct measurements of the subunit IV synthesis rate. Thus, both MCD1 and MCD2 may participate in both chloroplast RNA stability and translation initiation.

Citations

Jan 25, 2005·Plant Physiology·Linda A RymarquisDavid B Stern
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Linda A RymarquisDavid B Stern
Mar 1, 2009·American Journal of Botany·Joshua J BaeckerMargaret J Hollingsworth
Sep 7, 2005·Photosynthesis Research·David L Herrin, Jöerg Nickelsen
May 18, 2004·The Plant Journal : for Cell and Molecular Biology·Lina Lezhneva, Jörg Meurer

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