PMID: 8592644Sep 29, 1995Paper

A P2 purinoceptor activated by ADP in rat medullar neurons

Neuroscience Letters
Y IkeuchiY Okada

Abstract

ADP evoked outwardly rectifying potassium currents with a latency of 0.6 s in cultured rat medullar neurons. Purinoceptor agonists, such as 2-methylthio ATP (2-MeSATP), ATP, AMP, alpha,beta-methylene ATP (alpha,beta-MeATP), and UTP, produced similar outward currents with the order of their potencies for current amplitudes: 2-MeSATP > ADP > ATP > or = alpha,beta-MeATP > or = AMP > UTP. This order corresponds to that for a subtype of P2Y purinoceptors. ADP-evoked currents were fully blocked by a broad G-protein inhibitor, guanosine-5'-O-(2-thiodiphosphate) (GDP beta S), whereas a G(i)/G(o)-protein inhibitor, pertussis toxin (PTX) had no effect. The currents were not affected by a phospholipase C (PLC) inhibitor, neomycin. Furthermore, a selective protein kinase C inhibitor, GF109203X or a selective cAMP-dependent protein kinase inhibitor, H-89 showed no effect on the currents. These results suggest that ADP activates the potassium channel via a P2Y purinoceptor linked to a PTX-insensitive G-protein and its channel regulation may be due to a direct action of the G-protein beta gamma subunits regardless of second messenger signaling cascades. Additionally, ADP enhanced intracellular free Ca2+ concentration ([Ca2+]i) both in the pre...Continue Reading

References

Apr 1, 1991·Trends in Pharmacological Sciences·S E O'ConnorP Leff
Jan 1, 1985·General Pharmacology·G Burnstock, C Kennedy
Jan 15, 1986·The Biochemical Journal·J L Gordon
Mar 1, 1994·Trends in Pharmacological Sciences·E A BarnardT E Webb
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Citations

Feb 15, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Laszlo KölesPeter Illes
Apr 29, 1996·European Journal of Pharmacology·Y Ikeuchi, T Nishizaki
Jan 8, 2004·European Journal of Pharmacology·Peter Illes, J Alexandre Ribeiro
Nov 19, 1997·Neuropharmacology·J SimonE A Barnard
Dec 1, 1996·Journal of Autonomic Pharmacology·P IllesW Nörenberg
Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock

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