A pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig

Structure
C Withers-MartinezC Cambillau

Abstract

The guinea pig pancreatic lipase-related protein 2 (GPLRP2) differs from classical pancreatic lipases in that it displays both lipase and phospholipase A1 activities; classical pancreatic lipases have no phospholipase activity. The sequence of GPLRP2 is 63 % identical to that of human pancreatic lipase (HPL), but the so-called lid domain, is much reduced in GPLRP2. A phospholipase A1 from hornet venom (Dolml PLA1) is very similar to HPL and GPLRP2 but is devoid of lipase activity; Dolml PLA1 also contains a reduced lid domain and lacks a region termed the beta9 loop, which is located in the vicinity of the HPL and GPLRP2 active sites. The structure determination of a chimera of GPLRP2 and HPL and domain building of Dolml PLA1 were undertaken to gain a better understanding of the structural parameters responsible for the differences in lipase versus phospholipase activity among these structurally related enzymes. The crystal structure of a chimeric mutant of GPLRP2, consisting of the catalytic domain of GPLRP2 and the C-terminal domain of HPL, has been solved and refined to 2.1 A resolution. This enzyme belongs to the alpha/beta hydrolase fold family and shows high structural homology with classical pancreatic lipases. The activ...Continue Reading

References

Apr 1, 1992·Protein Engineering·D L OllisJ Schrag
Sep 10, 1992·Nature·H van TilbeurghC Cambillau
Jan 1, 1991·Methods in Enzymology·H M Verheij, G H de Haas
Feb 22, 1990·Nature·F K WinklerW Hunziker
Apr 20, 1990·European Journal of Biochemistry·A McPherson
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T G KirchgessnerA Andalibi
Jan 5, 1989·Journal of Molecular Biology·D LombardoC Cambillau
May 1, 1985·The Journal of Allergy and Clinical Immunology·T P KingL Kochoumian
Apr 24, 1968·Biochimica Et Biophysica Acta·G H de HaasL L van Deenen
Jan 19, 1971·Biochimica Et Biophysica Acta·M F MayliéP Desnuelle
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Feb 23, 1981·Biochimica Et Biophysica Acta·J FauvelL Douste-Blazy
Oct 23, 1981·Biochimica Et Biophysica Acta·J FauvelL Douste-Blazy
Mar 15, 1994·Biochemistry·K ThirstrupF Carrière
May 20, 1994·Journal of Molecular Biology·Y BourneC Cambillau
Jan 1, 1994·International Archives of Allergy and Immunology·D R Hoffman
Jan 11, 1994·Biochemistry·C MartinezC Cambillau
Aug 16, 1996·Biochimica Et Biophysica Acta·L AnderssonR Verger
Dec 1, 1958·Biochimica Et Biophysica Acta·L SARDA, P DESNUELLE

❮ Previous
Next ❯

Citations

Jan 15, 2011·Pharmaceutical Research·Vincent DelormeJean-François Cavalier
Aug 28, 1998·Chemistry and Physics of Lipids·J W SimonsH M Verheij
Jul 19, 2000·Enzyme and Microbial Technology·A L PaivaF X Malcata
Jan 11, 2001·Biochimica Et Biophysica Acta·A Svendsen
Nov 7, 1998·Biochimica Et Biophysica Acta·F CarrièreR Verger
Nov 26, 1999·Biochimica Et Biophysica Acta·H van TilbeurghF Carrière
Nov 26, 1999·Biochimica Et Biophysica Acta·S Longhi, C Cambillau
May 14, 2005·Journal of Virology·Jeremy P KamilHsing-Jien Kung
Feb 23, 2011·International Journal of Molecular Sciences·Gregory S Richmond, Terry K Smith
Jan 29, 2014·Applied Microbiology and Biotechnology·Christoph BuerthDenis Tielker
Oct 10, 2013·BioMed Research International·Neelam GurungVivek Rai
Sep 16, 2014·Journal of Molecular Biology·Alain RousselChristian Cambillau
Nov 17, 2012·European Journal of Medicinal Chemistry·Vanessa PointJean-François Cavalier
Nov 26, 2015·Insect Biochemistry and Molecular Biology·Ming-Hon HouAndrew H-J Wang
Jan 20, 2010·Biochimica Et Biophysica Acta·Sawsan AmaraFrédéric Carrière
Sep 8, 2015·Biochimie·Serdar ÜlkerJean-François Cavalier
Nov 25, 2014·Scientific Reports·Hiroshi DoiKunihiro Yoshida
Jan 24, 2007·Molecular Microbiology·Gregory S Richmond, Terry K Smith
Sep 29, 2004·Biochimica Et Biophysica Acta·Josiane De CaroAlain De Caro
Aug 26, 2006·Biochimica Et Biophysica Acta·Ahmed AloulouFrédéric Carrière
Feb 17, 2006·Protein Expression and Purification·Ahmed AloulouFrédéric Carrière
Nov 20, 2012·Toxicon : Official Journal of the International Society on Toxinology·Sophida SukprasertSakda Daduang
Dec 26, 2013·Biochimica Et Biophysica Acta·Ahmed AloulouAbdelkarim Abousalham
May 8, 2016·Biochimica Et Biophysica Acta·Neshatul Haque, N Prakash Prabhu
Aug 21, 2016·European Journal of Medicinal Chemistry·Vanessa PointJean-François Cavalier
Mar 5, 1999·Journal of Molecular Biology·A Kasuya, J M Thornton
May 20, 2016·Journal of Lipid Research·Meddy El AlaouiAbdelkarim Abousalham
Sep 8, 2006·Journal of Lipid Research·Abir Ben BachaHafedh Mejdoub
Nov 21, 1998·The Journal of Biological Chemistry·A RousselM Lowe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.