A panel of human neutralizing mAbs targeting SARS-CoV-2 spike at multiple epitopes

Nature Communications
Tal Noy-PoratRonit Rosenfeld

Abstract

The novel highly transmissible human coronavirus SARS-CoV-2 is the causative agent of the COVID-19 pandemic. Thus far, there is no approved therapeutic drug specifically targeting this emerging virus. Here we report the isolation and characterization of a panel of human neutralizing monoclonal antibodies targeting the SARS-CoV-2 receptor binding domain (RBD). These antibodies were selected from a phage display library constructed using peripheral circulatory lymphocytes collected from patients at the acute phase of the disease. These neutralizing antibodies are shown to recognize distinct epitopes on the viral spike RBD. A subset of the antibodies exert their inhibitory activity by abrogating binding of the RBD to the human ACE2 receptor. The human monoclonal antibodies described here represent a promising basis for the design of efficient combined post-exposure therapy for SARS-CoV-2 infection.

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Citations

Dec 18, 2020·Nature Communications·Yfat Yahalom-RonenTomer Israely
Feb 16, 2021·ACS Omega·Ofir SchusterLiron Feldberg
Dec 16, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Lara Danziger-IsakovMartina Sester
Mar 7, 2021·Virology·James Brett CaseMichael S Diamond
Mar 16, 2021·Emergent Materials·Praopim LimsakulItthipon Jeerapan
Mar 27, 2021·Analytical and Bioanalytical Chemistry·Moria Barlev-GrossAdva Mechaly
Feb 12, 2021·The Journal of Clinical Investigation·Clinton O OgegaJustin R Bailey
Apr 4, 2021·International Journal of Molecular Sciences·Linhua TianLili Wang
Apr 27, 2021·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Deepak KumarNagendra R Hegde
May 1, 2021·Journal of Clinical Medicine·Krešimir PavelićNandu Goswami
May 1, 2021·Antibody Therapeutics·Donovan GuttieresStacy L Springs
May 1, 2021·International Journal of Molecular Sciences·Chih-Wei Lin, Richard A Lerner
May 11, 2021·Human Genomics·Giuseppe NovelliJuergen K V Reichardt
May 28, 2021·Scientific Reports·Margherita PassarielloClaudia De Lorenzo
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alon Ben DavidAmram Torgeman
Jul 3, 2021·Viruses·Esen SokulluBenoit Coulombe
Jun 18, 2021·Frontiers in Molecular Biosciences·Arnaud John Kombe KombeTengchuan Jin

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Methods Mentioned

BETA
phage display
ELISA
biolayer interferometry
glycosylation
PCR
biosensors

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