A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules

Frontiers in Genetics
Meihua LiangXiaoyi Huang

Abstract

Background: A liquid biopsy using circulating exosomal genetic materials provides new insights for thyroid cancer diagnosis. This study aimed to identify plasma-derived exosomal biomarkers that could be used for early detection of papillary thyroid carcinoma (PTC). Method: Exosomal miRNAs in plasma were isolated from patients with benign thyroid nodules and patients with PTC. Profiling of exosomal miRNA was performed using RNA sequencing (RNA-seq) to identify miRNA candidates and differentiate the benign from malignant. The validation cohort consisted of 30 patients with benign thyroid nodules, 35 PTC patients, and 31 healthy individuals. Real-time PCR was used to quantify the expression of miRNA candidates. The diagnostic potential of the candidates was evaluated by receiver operating characteristic (ROC) curves. Results: After RNA-seq, eight plasma exosomal miRNAs were selected as candidates. Further validation indicated that the levels of exosomal miR-16-2-3p, miR-223-5p, miR-34c-5p, miR-182-5p, miR-223-3p, and miR-146b-5p were significantly lower in nodules compared to healthy controls (p < 0.0001), whereas miR-16-2-3p and miR-223-5p were significantly higher in the PTC cases than in those with benign nodules (p < 0.05). RO...Continue Reading

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Citations

Nov 12, 2020·Cancers·Dana M HartlSophie Leboulleux
Dec 19, 2020·Frontiers in Oncology·Kaixiang FengDetao Yin
Apr 1, 2021·Seminars in Cancer Biology·Hong ZhangRui-Xin Lin
Aug 12, 2021·Endocrine·Francesca CapriglioneDiego Russo
Jan 15, 2022·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Z WenL Ni

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Datasets Mentioned

BETA
GSE130512

Methods Mentioned

BETA
biopsies
biopsy
Chips
PCR
chip
RNA-seq
Transmission electron microscopy
electrophoresis
Solexa Sequencing

Software Mentioned

ExoQuick
Perl
GraphPad
cBot Cluster Generation System
ENCORI
miRDeep
Bowtie
DESeq2
GraphPad Prism
miRDeep2

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