PMID: 9533658Apr 9, 1998Paper

A panel set for epitope analysis of myeloperoxidase (MPO)-specific antineutrophil cytoplasmic antibody MPO-ANCA using recombinant hexamer histidine-tagged MPO deletion mutants

Journal of Clinical Immunology
K TomizawaK Suzuki

Abstract

A major target protein of antineutrophil cytoplasmic antibody with a perinuclear staining pattern (P-ANCA) has been identified as myeloperoxidase (MPO). Recombinant deletion mutants of MPO, eight fragments of the heavy-chain subunit, and two fragments of the light chain subunit were expressed in E. coli using a pQE expression vector. The recombinant hexamer histidine-tagged fragments were partially purified as the denatured proteins on a Ni2+-charged nitrirotriacetic acid column. The recombinant fragments were reacted with a rabbit polyclonal antibody to human MPO in Western blotting. In addition, the reactivities of the proteins with MPO-ANCA-positive sera of four patients with renal diseases were examined by Western blotting. The profile of the reactivity showed that different sera recognized different sets of fragments of the heavy chain, whereas no serum reacted with the fragments of the light chain. These results indicate that the sera of patients with MPO-ANCA-positive diseases showed varied reactivities with the different fragments. Furthermore, an ELISA system using a set of the fragments completely purified by Sephacryl S-200HR column chromatography was established. The panel set is useful for subclassification of MPO-...Continue Reading

Citations

May 3, 2006·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Kazuo Suzuki
Mar 8, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Tomokazu NagaoKazuo Suzuki
Aug 24, 2004·Clinical and Experimental Immunology·Y M van der GeldC G M Kallenberg
Apr 4, 2013·The Journal of Clinical Investigation·Aleeza J RothRonald J Falk
May 20, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Akiko Ishida-OkawaraKazuo Suzuki
Dec 14, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·William F PendergraftGloria A Preston
Dec 21, 2007·Microbiology and Immunology·Kazuo SuzukiHiroshi Hashimoto
Apr 1, 2006·Critical Reviews in Clinical Laboratory Sciences·John G RoutsiasAthanasios G Tzioufas
Jun 9, 2000·Kidney International·C F FranssenJ W Tervaert
Mar 25, 2006·Kidney International·U ErdbrüggerP H Nachman
May 9, 2006·Journal of Autoimmune Diseases·Marjan C SlotJan Willem Cohen Tervaert

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