A paneukaryotic genomic analysis of the small GTPase RABL2 underscores the significance of recurrent gene loss in eukaryote evolution

Biology Direct
Marek EliášJan Tachezy

Abstract

The cilium (flagellum) is a complex cellular structure inherited from the last eukaryotic common ancestor (LECA). A large number of ciliary proteins have been characterized in a few model organisms, but their evolutionary history often remains unexplored. One such protein is the small GTPase RABL2, recently implicated in the assembly of the sperm tail in mammals. Using the wealth of currently available genome and transcriptome sequences, including data from our on-going sequencing projects, we systematically analyzed the phylogenetic distribution and evolutionary history of RABL2 orthologs. Our dense taxonomic sampling revealed the presence of RABL2 genes in nearly all major eukaryotic lineages, including small "obscure" taxa such as breviates, ancyromonads, malawimonads, jakobids, picozoans, or palpitomonads. The phyletic pattern of RABL2 genes indicates that it was present already in the LECA. However, some organisms lack RABL2 as a result of secondary loss and our present sampling predicts well over 30 such independent events during the eukaryote evolution. The distribution of RABL2 genes correlates with the presence/absence of cilia: not a single well-established cilium-lacking species has retained a RABL2 ortholog. However...Continue Reading

References

Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·G W SaundersR A Andersen
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 31, 2000·Journal of Molecular Biology·C NotredameJ Heringa
Dec 26, 2001·Bioinformatics·H Shimodaira, M Hasegawa
Mar 5, 2002·American Journal of Human Genetics·Christa Lese MartinDavid H Ledbetter
Mar 28, 2002·Journal of Molecular Biology·Detlef D LeipeL Aravind
Jun 25, 2002·Systematic Biology·Hidetoshi Shimodaira
Mar 29, 2003·Journal of Molecular Evolution·Thomas Cavalier-Smith, Ema E-Y Chao
Sep 16, 2004·Science's STKE : Signal Transduction Knowledge Environment·John Colicelli
Apr 7, 2005·Molecular and Biochemical Parasitology·John P AckersMark C Field
Jun 16, 2005·Experimental Parasitology·Yumiko Saito-NakanoTomoyoshi Nozaki
Jul 7, 2005·The Journal of Cell Biology·Gregory J PazourGeorge B Witman
Jul 25, 2006·Trends in Genetics : TIG·Peter N InglisMichel R Leroux
Oct 20, 2006·Nature·Timothy Y JamesRytas Vilgalys
Jan 16, 2007·Science·Jane M CarltonPatricia J Johnson
Feb 6, 2007·Current Biology : CB·Hongmin QinJoel Rosenbaum
Apr 7, 2007·American Journal of Respiratory Cell and Molecular Biology·Andrea J RossRobert B Devlin
May 10, 2007·Developmental Cell·Tamara CasparyKathryn V Anderson
May 15, 2007·Molecular & Cellular Proteomics : MCP·Qin LiuEric A Pierce
Oct 13, 2007·Science·Sabeeha S MerchantArthur R Grossman
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Peter N InglisJonathan M Scholey
Feb 26, 2009·Journal of Cell Science·Christine AdhiamboPhilippe Bastin
Jun 10, 2009·Bioinformatics·Salvador Capella-GutiérrezToni Gabaldón
Apr 15, 2010·Journal of Cell Science·Matthew E HodgesKeith Gull
Jun 10, 2010·The Journal of Cell Biology·Yujie LiJinghua Hu
Feb 22, 2011·Protein & Cell·Yujie Li, Jinghua Hu
Apr 15, 2011·Biology of the Cell·Yi Shan LimBor Luen Tang
Jun 17, 2011·Annual Review of Biochemistry·Alfred Wittinghofer, Ingrid R Vetter
Jun 17, 2011·BMC Research Notes·Jennifer H Lumb, Mark C Field
Jun 21, 2011·Annual Review of Microbiology·Jennifer H Wisecaver, Jeremiah D Hackett
Jun 21, 2011·Small GTPases·Teunis J P van DamBerend Snel
Nov 17, 2011·BMC Evolutionary Biology·Satoshi SekimotoMary L Berbee
Nov 17, 2011·Genes & Development·Kevin J WrightPeter K Jackson

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Citations

May 5, 2017·Annual Review of Biochemistry·Michael P Rout, Mark C Field
Mar 29, 2018·Scientific Reports·Kristína ZáhonováMarek Eliáš
Oct 27, 2017·Small GTPases·Oliver E BlacqueStefanie Kuhns
Apr 22, 2017·Molecular Biology of the Cell·Yuya NishijimaKazuhisa Nakayama
Jun 21, 2018·PeerJ·Alexandra M Hernandez, Joseph F Ryan
Jan 24, 2017·PLoS Pathogens·Cordula M BoehmMark C Field
Jul 12, 2021·Genome Biology and Evolution·Romana VargováMarek Eliáš

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Datasets Mentioned

BETA
AF234189.1
AFZ78866.1
AEIE00000000.1
KU522217-KU522224

Methods Mentioned

BETA
GTPases
GTPase

Software Mentioned

blastn
PANTHER
SYSCILIA
BLAST
Quick2D
tblastn
HPC BlackBox
HMMER
blastp
trimAl

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