PMID: 8588735Aug 1, 1995Paper

A pAO1-encoded molybdopterin cofactor gene (moaA) of Arthrobacter nicotinovorans: characterization and site-directed mutagenesis of the encoded protein

Archives of Microbiology
C MenéndezR Brandsch

Abstract

A gene homologous to moaA, the gene responsible for the expression of a protein involved in an early step in the synthesis of the molybdopterin cofactor of Escherichia coli, was found to be located 2.7-kb upstream of the nicotine dehydrogenase (ndh) operon on the catabolic plasmid pAO1 of Arthrobacter nicotinovorans. The MoaA protein, containing 354 amino acids, migrated on an SDS-polyacrylamide gel with an apparent molecular weight of 40,000, in good agreement with the predicted molecular weight of 38,880. The pAO1-encoded moaA gene from A. nicotinovorans was expressed in E. coli as an active protein that functionally complemented moaA mutants. Its deduced amino acid sequence shows 43% identity to the E. coli MoaA, 44% to the NarAB gene product from Bacillus subtilis, and 42% to the gene product of two contiguous ORFs from Methanobacterium formicicum. N-terminal sequences, including the motif CxxxCxYC, are conserved among the MoaA and NarAB proteins. This motif is also present in proteins involved in PQQ cofactor synthesis in almost all the NifB proteins reported so far and in the fixZ gene product from Rhizobium leguminosarum. Mutagenesis of any of these three conserved cysteine residues to serine abolished the biological act...Continue Reading

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Citations

Oct 27, 2001·Applied and Environmental Microbiology·M SabatyA Vermeglio
Nov 24, 2007·FEMS Microbiology Reviews·Mercedes MaquedaManuel Martínez-Bueno
Apr 9, 2001·The Journal of Biological Chemistry·K MakdessiA Pich
Mar 14, 2002·The Journal of Biological Chemistry·Petra HänzelmannRalf R Mendel
Jun 8, 2004·The Journal of Biological Chemistry·Petra HänzelmannHermann Schindelin
Aug 10, 1999·Journal of Bacteriology·A SiedowB Friedrich
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·W G Zumft
Jan 31, 2014·Chemical Reviews·Joan B BroderickEric M Shepard

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