A paradoxical pro-apoptotic effect of thrombin on smooth muscle cells

Experimental Cell Research
Patrick RossignolOlivier Meilhac

Abstract

Whereas thrombin (below 10 nM) is a potent mitogen, recent studies report that exposure to higher doses of thrombin could lead to apoptosis of neurons and tumor cells. Our results show that prolonged exposure (> or = 24 h) to thrombin (50-100 nM) exerts a pro-apoptotic effect on cultured vascular smooth muscle cells (VSMCs). This phenomenon depends on thrombin serine-protease activity but is independent of PAR-1 and -4 activation and subsequent signaling. The parallel occurrence of cell retraction and cleavage of fibronectin suggests that thrombin-induced apoptosis is consecutive to pericellular proteolysis. These data point to a new potential action of thrombin in the cardiovascular system.

References

Dec 28, 1989·Gene·J SommerD Monard
Jan 1, 1986·Annals of the New York Academy of Sciences·D F Mosher, E D Thompson
Dec 1, 1984·The Biochemical Journal·K TakahashiD A Walz
Dec 1, 1993·The Journal of Cell Biology·R Bar-ShavitI Vlodavsky
Aug 1, 1996·The Journal of Cell Biology·S M FrischP Y Chan-Hui
Mar 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·Z S GalisP Libby
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W F XuD C Foster
Sep 3, 1998·Molecular Biology of the Cell·V Vouret-CraviariE Van Obberghen-Schilling
Feb 22, 2000·The Journal of Biological Chemistry·S R Adderley, D J Fitzgerald
Feb 14, 2002·Biochemistry. Biokhimii︠a︡·N V BogatchevaA D Verin
Nov 12, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·El Mostafa MtairagJean-Baptiste Michel
Jan 14, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Marie-Christine BoutonMartine Jandrot-Perrus
May 10, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Olivier MeilhacEduardo Anglés-Cano
May 31, 2003·The Journal of Biological Chemistry·Zhiming SuoBarry W Festoff
Oct 11, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Jean-Baptiste Michel

❮ Previous
Next ❯

Citations

Mar 25, 2006·Molecular Human Reproduction·Frank A HillsJan J Brosens
May 7, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guadalupe Ortiz-MuñozOlivier Meilhac
Feb 5, 2005·Médecine sciences : M/S·Olivier Meilhac, Jean-Baptiste Michel
Dec 9, 2015·Journal of Molecular and Cellular Cardiology·Mikhail A KolpakovAbdelkarim Sabri
Jun 30, 2009·The Annals of Thoracic Surgery·Peter RaivioJari Petäjä
May 15, 2009·The FEBS Journal·Pierangela TottaAntonio Facchiano
Sep 14, 2013·Microscopy Research and Technique·Carlos E Fonseca-AlvesRenee Laufer-Amorim
Jan 12, 2012·Blood·Marie-Christine BoutonMartine Jandrot-Perrus
Apr 1, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Jose Luis Martin-VenturaOlivier Meilhac
Sep 23, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Florian BeaErwin Blessing
Nov 3, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Laurent CalvierNatalia López-Andrés
Mar 11, 2010·Journal of Hypertension·Natalia Lopez-AndresPatrick Rossignol

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.