A partition-type tubular scaffold loaded with PDGF-releasing microspheres for spinal cord repair facilitates the directional migration and growth of cells

Neural Regeneration Research
Xue ChenXiao-Dong Wang

Abstract

The best tissue-engineered spinal cord grafts not only match the structural characteristics of the spinal cord but also allow the seed cells to grow and function in situ. Platelet-derived growth factor (PDGF) has been shown to promote the migration of bone marrow stromal cells; however, cytokines need to be released at a steady rate to maintain a stable concentration in vivo. Therefore, new methods are needed to maintain an optimal concentration of cytokines over an extended period of time to effectively promote seed cell localization, proliferation and differentiation. In the present study, a partition-type tubular scaffold matching the anatomical features of the thoracic 8-10 spinal cord of the rat was fabricated using chitosan and then subsequently loaded with chitosan-encapsulated PDGF-BB microspheres (PDGF-MSs). The PDGF-MS-containing scaffold was then examined in vitro for sustained-release capacity, biocompatibility, and its effect on neural progenitor cells differentiated in vitro from multilineage-differentiating stress-enduring cells (MUSE-NPCs). We found that pre-freezing for 2 hours at -20°C significantly increased the yield of partition-type tubular scaffolds, and 30 μL of 25% glutaraldehyde ensured optimal crossli...Continue Reading

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Citations

Dec 7, 2019·Stem Cell Reviews and Reports·Jiaying YuanXuehong Liu
Apr 26, 2019·Frontiers in Cellular Neuroscience·A BoeckerL Harhaus
May 21, 2020·Frontiers in Bioengineering and Biotechnology·Doddy Denise Ojeda-HernándezJuan C Mateos-Díaz
Apr 10, 2019·Neural Regeneration Research·Shuo LiuBin Wang

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Methods Mentioned

BETA
ELISA
density gradient centrifugation
fluorescence microscopy
Light microscopy
Scanning electron microscopy

Software Mentioned

GraphPad
GraphPad Prism

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