A paucity of structural integrity in cloned porcine blastocysts produced in vitro

Theriogenology
Deog-Bon KooYong-Mahn Han

Abstract

The structural integrity of blastocyst stage embryos, consisting of the inner cell mass (ICM) and trophectoderm (TE) cells, is a prerequisite for normal development after implantation in mammals. In this study, allocation of nuclear transfer (NT)-derived porcine blastocysts to the ICM and to the TE cells was examined and compared with IVF- and in vivo-derived embryos. NT-derived embryos had a lower developmental competence to the blastocyst stage than IVF-derived embryos (P < 0.05). Total cell number of NT-derived blastocysts was inferior to that of IVF-derived embryos (P < 0.05), although no difference was detected between the two groups in the ratio of ICM to total cells. However, in vivo-derived blastocysts had a higher proportion of ICM to total cells compared with in vitro-produced embryos (P < 0.01). To investigate what proportions of in vitro-produced porcine embryos represent normal structural integrity, differentially-stained blastocysts were individually classified into three presumptive groups (I: <20%; II: 20-40%; III: >40%) according to the ratio of ICM to total cells. Low proportions of NT- (12.5%, 7/56) and IVF-derived blastocysts (15.8%, 9/57) were assigned to Group II, presumptively having a normal range of str...Continue Reading

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Citations

Apr 3, 2013·Reproduction, Fertility, and Development·Chawalit SiriboonJyh-Cherng Ju
Mar 24, 2007·The Journal of Reproduction and Development·Kun ZhangNing Li
Aug 24, 2007·Reproduction, Fertility, and Development·Gabriela F Mastromonaco, W Allan King
Oct 3, 2007·Cloning and Stem Cells·Nadine HornenHeiner Niemann
Apr 19, 2007·Molecular Reproduction and Development·Sang-Goo LeeChang-Kyu Lee
Aug 7, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jung Sun ParkYong-Kook Kang
Jul 4, 2006·Molecular & Cellular Proteomics : MCP·Jung-Il ChaeKweon Yu

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