A PCR-based strategy to detect the common severe determinants of alpha thalassaemia

British Journal of Haematology
D K BowdenD R Higgs


A rapid and inexpensive polymerase chain reaction (PCR) based strategy is described which detects the three common, severe alpha thalassaemia determinants observed in southeast Asia (--SEA) and the Mediterranean (--MED and -(alpha)20.5). Oligonucleotide primers have been chosen which allow specific identification of both normal (alpha alpha) and abnormal (--) chromosomes using identical conditions in either the same or parallel PCR reactions. This strategy should be useful in the development of screening programmes to identify carriers of alpha thalassaemia (--/alpha alpha) and prenatal diagnosis of the Hb Bart's hydrops fetalis syndrome (--/--) for those populations in which this represents a major cause of perinatal death.


Jan 1, 1979·Hemoglobin·P WasiP Winichagoon
Jun 1, 1991·American Journal of Medical Genetics·T M KoT Y Lee
Oct 1, 1990·British Journal of Haematology·C DodéRajagopal Krishnamoorthy
Nov 1, 1990·Clinical Genetics·R GalanelloA Cao
Feb 27, 1988·Lancet·C KattamisA Metaxotou-Mavrommati
Jul 1, 1987·Human Genetics·M H BreuningP L Pearson
Dec 1, 1988·Journal of Medical Genetics·V J BuckleD J Weatherall
May 1, 1986·The Journal of Pediatrics·D W BianchiL Wolfe
Jul 1, 1986·American Journal of Hematology·E KanavakisC Kattamis
Jul 1, 1986·British Journal of Haematology·C VelatiG Fiorelli
Feb 25, 1986·Nucleic Acids Research·R C HardisonC W Schmid
Jul 1, 1985·British Journal of Obstetrics and Gynaecology·S T LiangD Todd
Jul 1, 1974·Clinical Endocrinology·I M HoldawayT Chard
Jul 1, 1968·The Journal of Pediatrics·B ThumasathitM Mangkornkanok
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L PressleyD J Weatherall
Jan 1, 1981·British Journal of Haematology·T SophocleousD J Weatherall
Mar 1, 1982·Journal of Periodontal Research·F AbbasU van der Velden
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·D J Weatherall
Jul 26, 1980·British Medical Journal·C KattamisD J Weatherall
Jul 31, 1965·British Medical Journal·M P DIAMONDA PARKER
Mar 5, 1960·Nature·L E LIE-INJO, B H JO


May 22, 2001·Prenatal Diagnosis·M KleanthousM Angastiniotis
Oct 22, 2003·Molecular Genetics and Metabolism·Urvashi BhardwajEdward R B McCabe
Mar 1, 1993·Baillière's Clinical Haematology·A Cao, M C Rosatelli
Mar 1, 1995·British Journal of Haematology·E BaysalT H Huisman
Jul 21, 1998·Annals of the New York Academy of Sciences·F KutlarA Kutlar
Dec 1, 1996·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·W W van SolingeR J Kraaijenhagen
Dec 14, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Karen M K de VooghtWouter W van Solinge
Nov 23, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Luksana MakonkawkeyoonSanit Makonkawkeyoon
Oct 24, 2009·Haematologica·Luksana MakonkawkeyoonSanit Makonkawkeyoon
Dec 17, 2009·European Journal of Applied Physiology·Imed TouhamiDaniel Le Gallais
Jan 17, 2009·Journal of Genetics·Hajer SialaSlaheddine Fattoum
Aug 20, 2015·Hemoglobin·Reza AlibakhshiSamaneh Shafieenia
May 1, 2010·Expert Opinion on Medical Diagnostics·John M Old, Shirley Henderson
Mar 4, 2006·British Journal of Haematology·Francisco J C PereiraLuísa Romão
Jan 23, 2003·Reproductive Biomedicine Online·Wirawit PiyamongkolDagan Wells
Nov 15, 2011·Hemoglobin·Erol Baysal
Aug 27, 2004·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Miho MaedaTeruo Harano
Apr 28, 2016·Hemoglobin·Ebrahim Miri-MoghaddamMorteza Karimipoor
Jun 1, 2016·The Indian Journal of Medical Research·Somphon PharephanLuksana Makonkawkeyoon
Sep 6, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M R WenningMaria F Sonati
May 30, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E BorgesMaria F Sonati
May 1, 1997·Hemoglobin·S el-Kalla, A R Mathews
Jul 1, 1997·Hemoglobin·S el-Kalla, A R Mathews
Jan 1, 1997·Hemoglobin·C OnerC Altay
May 14, 2008·Hemoglobin·Hamida Mesbah-AmrounJacques Elion
Dec 1, 1996·Prenatal Diagnosis·J M Old
Oct 1, 1996·American Journal of Hematology·A C KattamisP Fortina
Sep 18, 2001·American Journal of Hematology·J S WayeD H Chui
Sep 11, 2002·American Journal of Hematology·Urvashi BhardwajEdward R B McCabe
Sep 2, 1998·American Journal of Hematology·K LeeF Galactéros
Nov 14, 2018·Hemoglobin·Ilham YoussrySamia Rizk
Jan 1, 1993·Human Mutation·F F Chehab
Aug 6, 1998·American Journal of Hematology·V Oron-KarniD Rund
Dec 13, 2006·Clinical Chemistry·Sherry Sze Yee HoMahesh Choolani
Dec 5, 1998·American Journal of Hematology·R GalanelloA Cao
Jul 1, 1994·American Journal of Hematology·E Baysal, T H Huisman
May 9, 2007·Hemoglobin·Mehmet Akif Cürük
Oct 22, 2008·Hemoglobin·Mohammad T AkbariMarina Kleanthous
Sep 7, 2000·Hemoglobin·K KyriacouMarina Kleanthous
Mar 3, 2015·International Journal of Clinical Practice·Antonino GiambonaAurelio Maggio
Apr 10, 2002·Hemoglobin·Giuseppina LacerraClementina Caresti
Dec 2, 2010·Molecular Biology Reports·Aida ArabSirous Zeinali
Aug 12, 2003·Hemoglobin·Shaheen N KhanSheikh Riazuddin

Related Concepts

DNA Sequence
DNA, Double-Stranded
Mass Screening
Antenatal Screening Procedures
Immune Hydrops Fetalis
Nested Polymerase Chain Reaction

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