A pea chloroplast translation elongation factor that is regulated by abiotic factors

Biochemical and Biophysical Research Communications
B N SinghM K Reddy

Abstract

We report the cloning and characterization of both the cDNA (tufA) and genomic clones encoding for a chloroplast translation elongation factor (EF-Tu) from pea. The analysis of the deduced amino acids of the cDNA clone reveals the presence of putative transit peptide sequence and four GTP binding domains and two EF-Tu signature motifs in the mature polypeptide region. Using in vivo immunostaining followed by confocal microscopy pea EF-Tu was localized to chloroplast. The steady state transcript level of pea tufA was high in leaves and not detectable in roots. The expression of this gene is stimulated by light. The differential expression of this gene in response to various abiotic stresses showed that it is down-regulated in response to salinity and ABA and up-regulated in response to low temperature and salicylic acid treatment. These results indicate that regulation of pea tufA may have an important role in plant adaptation to environmental stresses.

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Citations

Jul 31, 2007·Photosynthesis Research·Julia Marín-NavarroStephen P Mayfield
Oct 25, 2007·Plant & Cell Physiology·Sun Jae KwonOhkmae K Park
Oct 14, 2008·Biochimica Et Biophysica Acta·Katharina DueckershoffJoerg Reinders
May 16, 2007·Plant Physiology and Biochemistry : PPB·Jocelyn R EasonPhillip West
Mar 19, 2016·Biochimica Et Biophysica Acta·Joanna MelonekKarin Krupinska
Aug 18, 2012·Plant Signaling & Behavior·Mohammad Amin OmidbakhshfardBernd Mueller-Roeber
May 10, 2017·International Journal of Molecular Sciences·Jinwei SuoShaojun Dai
Jan 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anket SharmaMarco Landi
Feb 25, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ana Campa, Juan José Ferreira
Nov 26, 2010·Journal of Agricultural and Food Chemistry·M Ángeles CastillejoJesús V Jorrín
Aug 22, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Rahma GoussiArafet Manaa

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