A peculiar molecular profile of umbilical cord-mesenchymal stromal cells drives their inhibitory effects on multiple myeloma cell growth and tumor progression

Stem Cells and Development
Sabino CiavarellaFranco Silvestris

Abstract

Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are under intensive investigation in preclinical models of cytotherapies against cancer, including multiple myeloma (MM). However, the therapeutic use of stromal progenitors holds critical safety concerns due to their potential MM-supporting activity in vivo. Here, we explored whether MSCs from sources other than BM, such as adipose tissue (AD-MSCs) and umbilical cord (UC-MSCs), affect MM cell growth in comparison to either normal (nBM-MSCs) or myelomatous marrow MSCs (MM-BM-MSCs). Results from both proliferation and clonogenic assays indicated that, in contrast to nBM- and MM-BM-MSCs, both AD and particularly UC-MSCs significantly inhibit MM cell clonogenicity and growth in vitro. Furthermore, when co-injected with UC-MSCs into mice, RPMI-8226 MM cells formed smaller subcutaneous tumor masses, while peritumoral injections of the same MSC subtype significantly delayed the tumor burden growing in subcutaneous plasmocytoma-bearing mice. Finally, both microarrays and ELISA revealed different expression of several genes and soluble factors in UC-MSCs as compared with other MSCs. Our data suggest that UC-MSCs have a distinct molecular profile that correlates with their intrinsi...Continue Reading

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Citations

Jul 28, 2016·Stem Cells and Development·Adam NowakowskiBarbara Lukomska
Oct 27, 2017·Oncotarget·Billy Samuel HillAntonella Zannetti

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Methods Mentioned

BETA
optical microscopy
ELISA
Antibody Array
optical
flow cytometry
Fluorescence

Software Mentioned

Excel
Prism
FlowJo
Ingenuity Pathway Analysis
FACSDiva
GraphPad
GeneSpring GX

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