A Penicillium expansum glucose oxidase-encoding gene, GOX2, is essential for gluconic acid production and acidification during colonization of deciduous fruit

Molecular Plant-microbe Interactions : MPMI
Shiri BaradDov Prusky

Abstract

Penicillium expansum, the causal agent of blue mold rot, causes severe postharvest maceration of fruit through secretion of total, d-gluconic acid (GLA). Two P. expansum glucose oxidase (GOX)-encoding genes, GOX1 and GOX2, were analyzed. GOX activity and GLA accumulation were strongly related to GOX2 expression, which increased with pH to a maximum at pH 7.0, whereas GOX1 was expressed at pH 4.0, where no GOX activity or extracellular GLA were detected. This differential expression was also observed at the leading edge of the decaying tissue, where GOX2 expression was dominant. The roles of the GOX genes in pathogenicity were further studied through i) development of P. expansum goxRNAi mutants exhibiting differential downregulation of GOX2, ii) heterologous expression of the P. expansum GOX2 gene in the nondeciduous fruit-pathogen P. chrysogenum, and iii) modulation of GLA production by FeSO(4) chelation. Interestingly, in P. expansum, pH and GLA production elicited opposite effects on germination and biomass accumulation: 26% of spores germinated at pH 7.0 when GOX activity and GLA were highest whereas, in P. chrysogenum at the same pH, when GLA did not accumulate, 72% of spores germinated. Moreover, heterologous expression o...Continue Reading

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Citations

Aug 7, 2013·Applied Microbiology and Biotechnology·Tianyuan ZhangHongye Li
Dec 20, 2012·Antioxidants & Redox Signaling·Noam AlkanDov Prusky
Mar 1, 2013·BMC Genomics·Naomi TrushinaBenjamin A Horwitz
Mar 29, 2014·International Journal of Food Microbiology·L VilanovaN Teixidó
Jun 19, 2013·Biotechnology and Bioengineering·Katja SchmitzStephan Noack
Oct 23, 2014·Molecular Plant-microbe Interactions : MPMI·Ana-Rosa BallesterToni Gabaldón
Apr 1, 2017·Critical Reviews in Food Science and Nutrition·Joanna TannousOlivier Puel
Apr 4, 2017·Database : the Journal of Biological Databases and Curation·Junyang YueYongsheng Liu
Feb 9, 2019·Die Naturwissenschaften·Željko SavkovićMilica Ljaljević Grbić
Sep 8, 2018·Frontiers in Microbiology·Yuxing WuLili Huang
Jul 25, 2019·Microorganisms·Ana-Rosa BallesterLuis González-Candelas
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Boqiang LiShiping Tian
Nov 26, 2021·Molecular Plant Pathology·Wenxiao JiaoMaorun Fu

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