Abstract
The study of peptide binding to HLA class II molecules has mostly concentrated on DR molecules. Since many autoimmune diseases show a primary association to particular DQ molecules rather than DR molecules, it is also important to study the peptide-binding properties of DQ molecules. Here we report a biochemical peptide-binding assay for the type I diabetes-associated DQ8, i.e. DQ (alpha1*0301, beta1*0302), molecule. Affinity-purified DQ8 molecules were tested in peptide-binding assays using a radiolabelled influenza haemagglutinin (Ha) peptide encompassing positions 255-271(Y) as an indicator peptide. The Ha 255-271(Y) peptide bound to DQ8 in a pH-dependent fashion showing optimal binding around pH 5. The association kinetics were relatively slow and the resulting complexes were heat labile. The specificity of peptide binding to DQ8 was investigated in competitive inhibition experiments with a panel of 43 peptides of different lengths and sequences. The DQ8 molecules showed a different pattern of peptide binding compared to a previously studied DQ2 molecule. Peptides derived from thyroid peroxidase, HLA-DQ(alpha1*0301), HLA-DQ(alpha1*0302), retinol receptor and p21ras were among the high-affinity binders, whereas peptides deri...Continue Reading
References
Nov 1, 1992·The Journal of Cell Biology·C V Harding, H J Geuze
Feb 21, 1991·Nature·P J PetersH J Geuze
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T H Watts, H M McConnell
Jul 1, 1986·The Journal of Experimental Medicine·B S NepomG T Nepom
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S BuusH M Grey
Aug 21, 1986·Nature·R N Germain
Dec 26, 1986·Cell·S BuusH M Grey
Jan 1, 1987·Immunogenetics·N TanigakiJ Cooper
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K OkadaJ L Strominger
Sep 2, 1985·Nature·B P BabbittE R Unanue
Apr 13, 1995·Biochimica Et Biophysica Acta·S BuusL O Pedersen
Aug 25, 1994·Nature·S Sadegh-NasseriR N Germain
Mar 17, 1994·Nature·L J SternD C Wiley
Mar 1, 1994·International Immunology·B H JohansenL M Sollid
Feb 1, 1994·European Journal of Immunology·A C OlsenS Buus
May 1, 1993·Diabetologia·E Thorsby, K S Rønningen
Jul 1, 1993·Nature·J H BrownD C Wiley
Jul 16, 1993·Cell·J HammerF Sinigaglia
Sep 1, 1993·Gastroenterology·L M Sollid, E Thorsby
Mar 1, 1996·The Journal of Experimental Medicine·W W KwokD M Koelle
Mar 1, 1997·Human Immunology·E Thorsby
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER
Citations
Aug 3, 2005·The Journal of Clinical Investigation·Anish SuriEmil R Unanue
Mar 26, 2008·Human Immunology·Elin BergsengLudvig M Sollid
May 18, 2005·Journal of Immunological Methods·Francisco A ChavesAndrea J Sant
Jul 17, 2007·Immunity·Kate N HendersonRobert P Anderson
Jan 9, 2018·Genes and Immunity·S RamgopalK Balakrishnan
Sep 3, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·John SidneyAlessandro Sette
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·John SidneyAlessandro Sette