A peptide fragment of beta cardiac myosin heavy chain (beta-CMHC) can provoke autoimmune myocarditis as well as the corresponding alpha cardiac myosin heavy chain (alpha-CMHC) fragment

Autoimmunity
K KohnoT Izumi

Abstract

The validity of the general belief that alpha cardiac myosin heavy chain (alpha-CMHC) is primarily responsible for causing experimental autoimmune myocarditis because of the more profound tolerance induction to beta-CMHC due to its expression during the embryonic stage has been examined. In order to completely avoid cross-contamination among components of the two myosin heavy chains, recombinant myosin fragments were synthesized in Escherichia coli using cDNA fragments of rat alpha- and beta-CMHC cloned by reverse transcription polymerase chain reaction (RT-PCR). Two fragments corresponding to amino acid residues 1107-1164 derived from alpha- and beta-heavy chains were equally capable of provoking severe myocarditis in Lewis rats when immunized in complete Freund's adjuvant. No significant differences in the severity, as judged from histological scoring, were observed between the diseases induced by the two different peptide fragments, indicating conclusively that beta-CMHC is as pathogenic as alpha-CMHC.

References

May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·V MahdaviB Nadal-Ginard
May 1, 1996·The Journal of Clinical Investigation·C L PummererN Neu

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Citations

Jan 12, 2011·Current Heart Failure Reports·Jose H Flores-ArredondoGuillermo Torre-Amione
Apr 3, 2008·Current Opinion in Cardiology·René CelisGuillermo Torre-Amione
Aug 21, 2012·Trends in Cardiovascular Medicine·HuiJuan Lv, Myra A Lipes
Jun 1, 2005·The American Journal of Cardiology·Guillermo Torre-Amione
Apr 4, 2009·Journal of the American College of Cardiology·Kirk U Knowlton, Byung-Kwan Lim
Apr 9, 2013·Current Protocols in Immunology·Jennifer M MyersMadeleine W Cunningham
Feb 4, 2006·Autoimmunity·Edecio Cunha-NetoDavid M Engman
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ya LiMadeleine W Cunningham

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