A peptide isolated by phage display binds to ICAM-1 and inhibits binding to LFA-1

Proteins
J K WelplyG F Casperson

Abstract

A mixed phage library containing random peptides from four to eight residues in length flanked by cysteine residues was screened using a recombinant soluble, form of human ICAM-1, which included residues 1-453, (ICAM-1(1-453)). Phage bound to immobilized ICAM-1(1-453) were eluted by three methods: (1) soluble ICAM-1(1-453), (2) neutralizing murine monoclonal antibody, (anti-ICAM-1, M174F5B7), (3) acidic conditions. After three rounds of binding and elution, a single, unique ICAM-1 binding phage bearing the peptide EWCEYLGGYLRCYA was isolated; the identical phage was selected with each method of elution. Attempts to isolate phage from non-constrained (i.e., not containing cysteines) libraries did not yield a phage that bound to ICAM-1. Phage displaying EWCEYLGGYLRCYA bound to immobilized ICAM-1(1-453) and to ICAM-1(1-185), a recombinant ICAM-1, which contains only the two amino-terminal immunoglobulin domains residing within residues 1-185. This is the region of the ICAM-1 that is bound by LFA-1. The phage did not bind to proteins other than ICAM-1. The phage bound to two ICAM-1 mutants, which contained amino acid substitutions that dramatically decreased or eliminated the binding to LFA-1. Studies were also performed with the c...Continue Reading

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Citations

Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·B A CohenR Brent
Aug 2, 2008·PLoS Computational Biology·Jihua HaoJames P Basilion
Apr 12, 2012·Protein Engineering, Design & Selection : PEDS·Olusegun A FagbohunValery A Petrenko
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Jan 15, 2008·Urologic Oncology·Frank AlexisOmid C Farokhzad
Sep 10, 2003·Biochemical and Biophysical Research Communications·Anna-Karine BélizaireValery Alakhov
May 7, 2015·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Jacob W MyersonVladimir R Muzykantov
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Aug 4, 2006·Cancer Research·David N KragChristian Thomas
Apr 25, 2000·Journal of Molecular Biology·D A KazminJ R Starkey

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