A peptide sequence of heparin/heparan sulfate (HP/HS)-interacting protein supports selective, high affinity binding of HP/HS and cell attachment.

The Journal of Biological Chemistry
S LiuD D Carson

Abstract

We previously have identified a novel cell surface heparan sulfate/heparin (HS/HP)-interacting protein (HIP) found in human uterine epithelia and a variety of other human epithelial and endothelial cells and cell lines (Liu, S., Smith, S. E., Julian, J., Rohde, L. H., Karin, N. J., and Carson, D. D. (1996) J. Biol. Chem. 271, 11817-11823; Rohde, L. H., Julian, J., Babaknia, A., and Carson, D. D. (1996) J. Biol. Chem. 271, 11824-11830). The amino acid sequence predicted for HIP revealed a potential HS/HP-binding motif. In the present studies, a synthetic peptide corresponding to this putative HS/HP-binding motif, HIP peptide, was synthesized and examined with regard to its HS/HP binding and cell attachment promoting activity. Results using solid phase binding assays demonstrate that HIP peptide binds HS/HP with high selectivity and has high affinity for bulk HP (50% saturation congruent with 300 nM) and even higher affinity for a subset of polysaccharides found in commercial [3H]HP (half-saturation congruent with 10 nM). Moreover, HIP peptide binds subsets of cell and extracellular matrix-associated HS and dermatan sulfate expressed by RL95 cells, a human uterine adenocarcinoma cell line. HIP peptide also binds a similar fractio...Continue Reading

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Citations

Oct 1, 2007·Frontiers of Medicine in China·Caixia LeiYinkun Liu
Aug 31, 2000·Genomics·C B Kirn-SafranD D Carson
Jan 23, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Catherine B Kirn-SafranDaniel D Carson
Apr 1, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Catherine B Kirn-SafranDaniel D Carson
Jun 4, 2011·Journal of Polymer Science. Part A, Polymer Chemistry·Linqing LiKristi L Kiick
Feb 14, 2006·Journal of Cellular Physiology·Jian-Jun LiuShing Chuan Hooi
Feb 27, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ulf Meyer-HoffertKatrin Pütsep
Sep 17, 1998·The Journal of Biological Chemistry·D E HokeD D Carson
May 23, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michal Jaron-MendelsonAngel Porgador

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