A Perfusion Bioreactor System for Cell Seeding and Oxygen-Controlled Cultivation of Three-Dimensional Cell Cultures

Tissue Engineering. Part C, Methods
Jakob SchmidRobert Huber

Abstract

Bioreactor systems facilitate three-dimensional (3D) cell culture by coping with limitations of static cultivation techniques. To allow for the investigation of proper cultivation conditions and the reproducible generation of tissue-engineered grafts, a bioreactor system, which comprises the control of crucial cultivation parameters in independent-operating parallel bioreactors, is beneficial. Furthermore, the use of a bioreactor as an automated cell seeding tool enables even cell distributions on stable scaffolds. In this study, we developed a perfusion microbioreactor system, which enables the cultivation of 3D cell cultures in an oxygen-controlled environment in up to four independent-operating bioreactors. Therefore, perfusion microbioreactors were designed with the help of computer-aided design, and manufactured using the 3D printing technologies stereolithography and fused deposition modeling. A uniform flow distribution in the microbioreactor was shown using a computational fluid dynamics model. For oxygen measurements, microsensors were integrated in the bioreactors to measure the oxygen concentration (OC) in the geometric center of the 3D cell cultures. To control the OC in each bioreactor independently, an automated f...Continue Reading

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Citations

Oct 22, 2019·Preparative Biochemistry & Biotechnology·Olga Nehir OztelErdal Karaoz
Dec 19, 2019·International Journal of Molecular Sciences·M Piñeiro-RamilS M Díaz-Prado
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Chrysanthi-Maria MoysidouRóisín Meabh Owens
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Tarun AgarwalTapas Kumar Maiti
Feb 13, 2021·Biofabrication·Valerio Luca MainardiMatteo Moretti
May 12, 2021·Journal of Biomechanical Engineering·Sophia M Mavris, Laura M Hansen
Jul 26, 2021·Bioprocess and Biosystems Engineering·Maria Elena LombardoVincenzo La Carrubba

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Methods Mentioned

BETA
microsensors
fluorescence microscopy

Software Mentioned

LabView
ANSYS CFX
Zen
ANSYS Workbench

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