PMID: 9555901Apr 29, 1998Paper

A period-extender gene, pex, that extends the period of the circadian clock in the cyanobacterium Synechococcus sp. strain PCC 7942

Journal of Bacteriology
S KutsunaM Ishiura

Abstract

We cloned the pS1K1 plasmid in the process of apparently "complementing" a circadian clock mutant of cyanobacterium Synechococcus sp. strain PCC 7942, SP22, which has a 22-h period (T. Kondo, N. F. Tsinoremas, S. S. Golden, C. H. Johnson, S. Kutsuna, and M. Ishiura, Science 266:1233-1236, 1994). Sequence analysis revealed that SP22 did not have a mutation in the genomic DNA segment carried on pS1K1, and the sp22 mutation was later found in a recently cloned new clock gene, kaiC. Therefore, the period-extender gene pex that was carried on pS1K1 was a suppressor gene for the sp22 mutation. The pex gene encoded a protein of 148 amino acid residues. No meaningful homologs were found in DNA or protein databases including the Synechocystis genome database. The pex gene was transcribed from 129 and 164 bp upstream of the translation initiation codon as 0.6-kb transcripts. The Pex protein was detected as a fusion protein with a molecular mass of 15 kDa by the epitope tag fusion method using a c-Myc epitope tag. Disruption of the pex gene in wild-type cells shortened the period of the rhythms by 1 h, although it did not affect other properties of the rhythms, whereas its overexpression extended the period by 3 h with a concomitant reduc...Continue Reading

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Citations

Dec 2, 2000·Plant & Cell Physiology·H Iwasaki, T Kondo
Oct 19, 2004·Annual Review of Microbiology·Patricia L Lakin-Thomas, Stuart Brody
Mar 19, 2005·The EMBO Journal·Natalia B IvlevaSusan S Golden
Nov 14, 2006·The Journal of Biological Chemistry·Kyouhei AritaToshiyuki Shimizu
Oct 8, 2015·Annual Review of Genetics·Ryan K ShultzabergerSusan S Golden
Mar 29, 2001·Journal of Bacteriology·T Mori, C H Johnson
Jul 25, 2000·Chronobiology International·M ZordanG Tosini
Apr 19, 2008·Cold Spring Harbor Symposia on Quantitative Biology·S S Golden
Nov 5, 1999·Annual Review of Microbiology·C H Johnson, S S Golden
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·T NishiwakiT Kondo
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Volodymyr DvornykEviatar Nevo
Sep 21, 2004·The Journal of Biological Chemistry·Fumio HayashiMasahiro Ishiura
Apr 19, 2008·Cold Spring Harbor Symposia on Quantitative Biology·S R Mackey
Nov 18, 2003·Annual Review of Genetics·J L DittyS S Golden
Feb 8, 2005·Journal of Molecular Evolution·Volodymyr Dvornyk
Apr 25, 2006·Molecular Microbiology·Xiaofan ZhangSusan S Golden
Aug 9, 2011·Essays in Biochemistry·Susan K Crosthwaite
Jun 30, 2012·Plant & Cell Physiology·Hiromitsu TanakaShinsuke Kutsuna
Sep 2, 2005·Genetica·Volodymyr Dvornyk, Bjarne Knudsen
Nov 27, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Shunsuke KurosawaMasahiro Ishiura
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi ItoHideo Iwasaki
Jul 16, 2011·Nature Reviews. Microbiology·Peter Lenz, Lotte Søgaard-Andersen
Jul 25, 2017·BMC Evolutionary Biology·Nicolas M SchmellingAnika Wiegard
May 29, 1999·Journal of Bacteriology·M KatayamaS S Golden
Jul 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Paul D Etter, Mani Ramaswami
Mar 6, 1999·Current Opinion in Microbiology·S S GoldenT Kondo
Apr 4, 2000·Current Opinion in Microbiology·H Iwasaki, J C Dunlap
May 14, 1999·Trends in Plant Science·T Kondo, M Ishiura
Apr 5, 2005·Journal of Bacteriology·Yota B KiyoharaTakao Kondo
Jun 12, 2002·Journal of Bacteriology·Usha NairSusan S Golden
Aug 21, 2007·Journal of Bacteriology·Shinsuke KutsunaMasahiro Ishiura
Dec 10, 2003·Current Opinion in Microbiology·Susan S Golden
Feb 13, 1999·Cell·J C Dunlap
Oct 12, 2010·Current Opinion in Genetics & Development·Guogang DongSusan S Golden

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