A Personal Glucose Meter for Label-Free and Washing-Free Biomolecular Detection

Analytical Chemistry
Jun Ki AhnHyun Gyu Park

Abstract

We developed a label-free and washing-free method for biomolecular detection using a personal glucose meter (PGM). ATP was selected as a model target, and cascade enzymatic reactions promoted by hexokinase and pyruvate kinase were adopted to link the amount of ATP to glucose that is detectable by a hand-held PGM. In principle, the presence of target ATP enables hexokinase to catalyze the conversion of glucose to glucose 6-phosphate by providing a phosphate group to glucose, and thus the amount of glucose is decreased in proportion to the amount of ATP. In addition, adenosine 5'-diphosphate (ADP), which is generated after hexokinase-catalyzed enzymatic reaction, is recovered to ATP by a pyruvate kinase enzyme. The regenerated ATP is again supplemented to catalyze multiple rounds of cascade enzymatic reactions, leading to signal amplification. As a result, the change of glucose amount that is inversely proportional to ATP amount is simply measured by a hand-held PGM. By employing this strategy, we successfully determined ATP down to 49 nM with high selectivity even in real samples such as tap water, human serum, and bovine urine. Importantly, the developed system does not require expensive modification and washing steps but is co...Continue Reading

References

Jan 1, 1982·The Journal of Membrane Biology·M Erecińska, D F Wilson
Oct 19, 2004·Journal of Neuroscience Methods·Jason A GruenhagenEdward S Yeung
Apr 12, 2007·Diabetes Technology & Therapeutics·Aaron E CarrollStephen M Downs
Mar 20, 2010·Biosensors & Bioelectronics·Yonghong WangXiaoqi Ni
Jun 4, 2013·Analytica Chimica Acta·Wimonsiri TedsanaWittaya Ngeontae
Sep 28, 2014·Journal of Alzheimer's Disease : JAD·Cheng ZhangJune Feng
May 12, 2015·Chemical Communications : Chem Comm·Ki Soo ParkHyun Gyu Park
Apr 8, 2016·Analytical and Bioanalytical Chemistry·Chao ZhuAiliang Chen

❮ Previous
Next ❯

Citations

Jan 31, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaomeng ZhouLi Shang
Jul 7, 2020·The Analyst·Hyo Yong KimHyun Gyu Park
Jun 11, 2019·Journal of Biological Engineering·Jun Ki AhnHyun Gyu Park
Jul 9, 2020·Chemical Communications : Chem Comm·Hyo Yong KimHyun Gyu Park
Sep 4, 2020·Analytical and Bioanalytical Chemistry·Wenguang ZhangJiayu Wan
Dec 11, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Prabhpreet SinghSatwinderjeet Kaur

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.