A personal perspective on high-content screening (HCS): from the beginning

Journal of Biomolecular Screening
D Lansing Taylor

Abstract

High-content screening (HCS) was introduced in 1997 based on light microscope imaging technologies to address the need for an automated platform that could analyze large numbers of individual cells with subcellular resolution using standard microplates. Molecular specificity based on fluorescence was a central element of the platform taking advantage of the growing list of reagent classes and the ability to multiplex. In addition, image analysis coupled to data management, data mining, and data visualization created a tool that focused on biological information and knowledge to begin exploring the functions of genes identified in the genomics revolution. This overview looks at the development of HCS, the evolution of the technologies, and the market up to the present day. In addition, the options for adopting uniform definitions is suggested along with a perspective on what advances are needed to continue building the value of HCS in biomedical research, drug discovery, and development and diagnostics.

References

Mar 1, 1990·International Journal of Radiation Biology·O YamamotoT Nomura
Jan 1, 1990·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·S S SachdevC N Desai
Nov 1, 1989·Journal of Cellular Physiology·G R BrightD L Taylor
Jan 1, 1989·Reproduction, Nutrition, Development·B Marquant-Le GuienneC Thibault
Feb 13, 2001·Current Opinion in Biotechnology·D L TaylorK A Giuliano
Apr 5, 2003·Science·Jason R SwedlowPeter K Sorger
Dec 24, 2003·Trends in Biotechnology·Vivek C AbrahamJeffrey R Haskins
Apr 20, 2004·Assay and Drug Development Technologies·Kenneth A GiulianoD Lansing Taylor
Oct 8, 2004·Nature Biotechnology·Leroy Hood, Roger M Perlmutter
Nov 13, 2004·Science·Zachary E PerlmanSteven J Altschuler
Apr 1, 2005·PLoS Biology·Masahiro TanakaCynthia L Adams
Nov 25, 2005·Assay and Drug Development Technologies·Kenneth A GiulianoD Lansing Taylor
Apr 4, 2006·Nature Reviews. Drug Discovery·Paul LangAlexander Scheer
Nov 18, 2006·Methods in Enzymology·Kenneth A GiulianoD Lansing Taylor
Mar 31, 2007·Drug Discovery Today·Wayne Materi, David S Wishart
Apr 3, 2007·Nature Methods·Lit-Hsin LooSteven J Altschuler
Jul 20, 2007·Nature Chemical Biology·Anne E Carpenter
Dec 11, 2007·Critical Reviews in Biochemistry and Molecular Biology·Jillian R BrownJeffrey D Esko
Dec 15, 2007·Nature Reviews. Drug Discovery·Andrew Bullen
Jan 18, 2008·Biophysical Journal·Javier SatulovskyYu-li Wang
Sep 12, 2008·Nature Reviews. Neuroscience·Mike Dragunow
Dec 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael D SlackSteven J Altschuler
Jun 18, 2009·Combinatorial Chemistry & High Throughput Screening·Rebecca J Critchley-ThorneWilma L Lingle
Jul 2, 2009·Nature Reviews. Drug Discovery·Yan FengJohn A Tallarico
Sep 22, 2009·Nature Methods·Lit-Hsin LooSteven J Altschuler
Nov 26, 2009·Combinatorial Chemistry & High Throughput Screening·Flora Tang, Jinghai J Xu
Dec 17, 2009·Journal of Biomolecular Screening·Nick Thomas
Jul 20, 2010·Journal of Biomolecular Screening·Kenneth A GiulianoPatricia A Johnston

❮ Previous
Next ❯

Citations

Jul 17, 2012·Journal of Laboratory Automation·Constantin RaduHakim Djaballah
May 2, 2013·PLoS Computational Biology·Fuhai LiStephen T C Wong
Dec 21, 2013·Biotechnology Journal·Camille PrévelMay C Morris
Mar 15, 2016·Expert Opinion on Drug Discovery·Ivan Fraietta, Fabio Gasparri
Mar 20, 2015·Archives of Toxicology·Laia TolosaM Teresa Donato
Aug 31, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Carolina Garcia-CantonClive Meredith
May 21, 2015·Experimental Biology and Medicine·Nina SenutovitchD Lansing Taylor
Jan 12, 2012·Journal of Biomolecular Screening·Frans CornelissenEmmanuel Gustin
Dec 18, 2013·Advanced Materials·Nekane Guarrotxena, Guillermo C Bazan
Oct 31, 2019·Archives of Toxicology·Shuaizhang Li, Menghang Xia
Oct 20, 2011·Journal of Cell Science·Mien-Chie Hung, Wolfgang Link

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
flow cytometry

Software Mentioned

HCS
NovelTech Systems
BioDx
JBS
Cellomics
ArrayScan

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.