A personalized microRNA microarray normalization method using a logistic regression model

Bioinformatics
Bin WangYaguang Xi

Abstract

MicroRNA (miRNA) is a set of newly discovered non-coding small RNA molecules. Its significant effects have contributed to a number of critical biological events including cell proliferation, apoptosis development, as well as tumorigenesis. High-dimensional genomic discovery platforms (e.g. microarray) have been employed to evaluate the important roles of miRNAs by analyzing their expression profiling. However, because of the small total number of miRNAs and the absence of well-known endogenous controls, the traditional normalization methods for messenger RNA (mRNA) profiling analysis could not offer a suitable solution for miRNA analysis. The need for the establishment of new adaptive methods has come to the forefront. Locked nucleic acid (LNA)-based miRNA array was employed to profile miRNAs using colorectal cancer cell lines under different treatments. The expression pattern of overall miRNA profiling was pre-evaluated by a panel of miRNAs using Taqman-based quantitative real-time polymerase chain reaction (qRT-PCR) miRNA assays. A logistic regression model was built based on qRT-PCR results and then applied to the normalization of miRNA array data. The expression levels of 20 additional miRNAs selected from the normalized li...Continue Reading

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Citations

Feb 5, 2013·Acta Pharmacologica Sinica·Guomin ShenYaguang Xi
Jan 10, 2013·Source Code for Biology and Medicine·Guy N BrockM Michele Pisano
Feb 13, 2013·BMC Medical Genomics·Rehman Qureshi, Ahmet Sacan
Oct 29, 2013·Microarrays·Bin Wang, Yaguang Xi
Mar 18, 2016·Journal of Radiation Research·Jennifer Y Y KwanFei-Fei Liu
Jun 4, 2011·PLoS Computational Biology·Daniel-Adriano SilvaXuhui Huang
Sep 1, 2016·Journal of Cellular Physiology·Shima Rastegar-PouyaniAbdulshakour Mohammadnia
Jan 1, 2014·Microarrays·Niccolò P BassaniElia M Biganzoli
Jul 25, 2017·Cold Spring Harbor Protocols·Assen RoguevNevan J Krogan

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