A PFG NMR experiment for translational diffusion measurements in low-viscosity solvents containing multiple resonances

Journal of Magnetic Resonance
Alana K Simorellis, Peter F Flynn

Abstract

Pulsed gradient simulated-echo (PGSE) NMR diffusion measurements provide a facile and accurate means for determining the self-diffusion coefficients for molecules over a wide range of sizes and conditions. The measurement of diffusion in solvents of low intrinsic viscosity is particularly challenging, due to the persistent presence of convection. Although convection can occur in most solvent systems at elevated temperatures, in lower viscosity solvents (e.g., short chain alkanes), convection may manifest itself even at ambient laboratory temperatures. In most circumstances, solvent suppression will also be required, and for solvents that have multiple resonances, effective suppression can likewise represent a substantial challenge. In this article, we report an NMR experiment that combines a double-stimulated echo PFG approach with a WET-based solvent suppression scheme that effectively and simultaneously address the issues of dynamic range and the deleterious effects of convection. The experiment described will be of general benefit to studies aimed at the characterization of diffusion of single molecules directly dissolved in low-viscosity solvents, and should also be of substantial utility in studies of supramolecular assemb...Continue Reading

References

May 1, 1994·Journal of Magnetic Resonance. Series B·R J OggJ S Taylor
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·A J WandP F Flynn
Jul 18, 2001·Journal of the American Chemical Society·C R BabuA J Wand
Jun 8, 2002·Biotechnology Progress·M JiménezM Pérez-Gilabert
Mar 1, 1985·Biotechnology Progress·K E Cöklen, T A Hatton

❮ Previous
Next ❯

Citations

Aug 22, 2009·Journal of Biomolecular NMR·Gang Zheng, William S Price
Feb 26, 2008·Journal of Biomolecular NMR·Wade D Van HornPeter F Flynn
Sep 30, 2005·Journal of the American Chemical Society·Wade D Van HornPeter F Flynn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.