A phage display system designed to detect and study protein-protein interactions

Molecular Microbiology
Catherine L BairSankar Adhya

Abstract

Analysing protein-protein interactions is critical in proteomics and drug discovery. The usage of 2-Hybrid (2lambda) systems is limited to an in vivo environment. We describe a bacteriophage 2-Hybrid system for studying protein interactions in vitro. Bait and prey are displayed as fusions to the surface of phage lambda that are marked with different selectable drug-resistant markers. An interaction of phages in vitro through displayed proteins allows bacterial infection by two phages resulting in double drug-resistant bacterial colonies at very low multiplicity of infections. We demonstrate interaction of the protein sorting signal Ubiquitin with the Vps9-CUE, a Ubiquitin binding domain, and by the interaction of (Gly-Glu)(4) and (Gly-Arg)(4) peptides. Interruptions of the phage interactions by non-fused (free) bait or prey molecules show how robust and unique our approach is. We also demonstrate the use of Ubiquitin and CUE display phages to find binding partners in a lambda-display library. The unique usefulness to 2lambda is also described.

References

Apr 12, 1973·Molecular & General Genetics : MGG·P Kourilsky
Jan 1, 1984·Molecular & General Genetics : MGG·E T Palva, T J Silhavy
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Sternberg, R H Hoess
Sep 13, 1996·Journal of Molecular Biology·Y G MikawaS Brenner
Nov 1, 1996·Analytical Biochemistry·J Zhang, S Lautar
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Jun 29, 1999·Nature Biotechnology·P S Chowdhury, I Pastan
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·J K JoungC O Pabo
Nov 17, 2001·Chemical Reviews·R H Hoess
Mar 9, 2002·Current Pharmaceutical Biotechnology·Ronald H Hoess
Jul 12, 2002·Proteomics·Daniel AuerbachIgor Stagljar
Sep 25, 2002·Journal of Molecular Biology·Carla CicchiniAlessandra Luzzago
Nov 14, 2002·Annual Review of Genetics·Donald L CourtLynn C Thomason
Jun 6, 2003·Cell·Gali PragJames H Hurley
Jun 26, 2003·Protein Science : a Publication of the Protein Society·Inbal HalperinRuth Nussinov
Nov 11, 2003·Journal of Molecular Biology·Amita GuptaVijay K Chaudhary
Oct 20, 2004·The Journal of Biological Chemistry·Mitchell HoIra Pastan
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Gali PragJames H Hurley
Mar 24, 2005·Bioscience, Biotechnology, and Biochemistry·Yoshihito SuzukiIsomaro Yamaguchi
Jul 5, 2005·Journal of Immunological Methods·M KirschM Hust
Jul 5, 2005·World Journal of Gastroenterology : WJG·Bing YuGuan-Xin Shen
Oct 15, 2005·Nucleic Acids Research·Christine N ZanghiStephen Dewhurst
Nov 1, 2005·Applied Biochemistry and Biotechnology·Mojca LunderNadja Plazar
Nov 16, 2005·Annual Review of Genetics·Amos B OppenheimSankar Adhya
Jan 25, 2007·Molecular Cell·Svetoslava D StamenovaLinda Hicke

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Trends in Microbiology
Lisa M MullenBrian Henderson
Biochemical and Biophysical Research Communications
Hui-Chu HsiehChin Yu
© 2021 Meta ULC. All rights reserved