A phenothiazine coumarin based ratiometric fluorescent probe for real-time detection of lysosomal hypochlorite in living cell and zebra fish.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Jiaojiao LiuXiangzhi Song

Abstract

Hypochlorite (ClO-), a type of reactive oxygen species (ROS), plays an essential role in complex biological systems. Real-time detection of the content and distribution of ClO- in cells or subcellular organelle is critically essential. In this paper, a lysosomal-targeted fluorescent probe, Cou-Lyso, was constructed for real-time detection of ClO- in a ratiometric manner, achieving high sensitivity with a low detection limit (0.58 μM). Upon reaction with ClO-, this probe was subjected to a significant fluorescence change from red emission (λmaxem = 610 nm) to green emission (λmaxem = 535 nm) with the ratio of I535 nm/I610 nm displaying a 76-fold enhancement from 0.04 to 3.03. The confocal imaging experiments for Cou-Lyso showed that this probe could detect ClO- in living cell and zebra fish. This probe has been successfully applied to stain lysosome and image lysosomal ClO- based on co-localization imaging experiments.

References

Sep 9, 2006·Cellular Signalling·Yann Wan YapNam Sang Cheung
May 3, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Marla J SteinbeckJavad Parvizi
Sep 3, 2008·Bioorganic & Medicinal Chemistry Letters·Aaron E AlbersChristopher J Chang
Jun 2, 2011·Chemical Society Reviews·Xiaoqiang ChenJuyoung Yoon
Dec 2, 2015·Chemical Communications : Chem Comm·Zheng Xu, Lin Xu
May 18, 2017·Cellular Immunology·Anna StrzepaBonnie N Dittel
Nov 8, 2017·International Journal of Biological Macromolecules·Magdalena BonclerCezary Watala
Jan 25, 2018·Chemical Communications : Chem Comm·Xingjiang LiuXiangzhi Song
Mar 31, 2018·Chemistry : a European Journal·Shichao WangXuanjun Zhang
Apr 6, 2018·Chemical Science·Peng WeiTao Yi
Jul 16, 2019·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·He MengShi-Li Shen
Nov 23, 2019·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Wenqiang ChenLei Yang
Feb 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hongde XiaoRuiyong Wang
Jul 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mingguang RenWeiying Lin
Jan 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Liang QiaoXiaoling Zhang

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