A phosphatase activity in Xenopus oocyte extracts preferentially dephosphorylates the MPM-2 epitope

FEBS Letters
S CheJian Kuang

Abstract

MPM-2 antigens are a large family of mitotic phosphoproteins that contain similar phosphoepitopes recognized by the anti-phosphoepitope antibody MPM-2 (MPM-2 epitopes). These proteins are phosphorylated during M phase induction and dephosphorylated from the onset of anaphase through interphase. Since biochemical characterization of the MPM-2 epitope phosphatase requires a specific assay for its activity, we tested different methods for measurement of the MPM-2 epitope phosphatase activity in crude cell lysates. First, an ELISA-based assay was designed that measured the phosphatase-induced reduction of the MPM-2 reactivity in crude M phase cell lysates. Using this assay to follow the phosphatase activity during sequential chromatography of Xenopus oocyte extracts, one predominant peak of phosphatase activity was detected which was separated from the majority of PP1 and PP2A activities. This phosphatase activity dephosphorylated the MPM-2 epitope on multiple MPM-2 antigens. The second method measured dephosphorylation of cdc25, a known MPM-2 antigen. Two major peaks of cdc25 dephosphorylating activities were detected during the sequential chromatography, one that copurified with the major peak of MPM-2 epitope phosphatase activit...Continue Reading

References

Nov 30, 1990·Cell·M J SolomonM W Kirschner
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J KuangP N Rao
Jan 1, 1988·Methods in Enzymology·P CohenH Y Tung
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F M DavisP N Rao
Nov 1, 1993·The Journal of Cell Biology·J Kuang, C L Ashorn
Feb 1, 1994·Molecular Biology of the Cell·J KuangJ E Penkala
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M WestendorfL Gerace
May 1, 1997·Current Biology : CB·P T StukenbergM W Kirschner

❮ Previous
Next ❯

Citations

Jul 14, 2011·Nature Reviews. Molecular Cell Biology·Claudia Wurzenberger, Daniel W Gerlich
Mar 12, 2010·Molecular Biology of the Cell·Chuan Fen WuJian Kuang
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S A InnocenteJ M Lee
Sep 2, 2006·Journal of Neurochemistry·D Allan ButterfieldRukhsana Sultana
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael L Goldberg
Sep 1, 2015·Cell·Brent R StockwellElise Jiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.