A phosphatidylinositol 3-kinase and phosphatidylinositol transfer protein act synergistically in formation of constitutive transport vesicles from the trans-Golgi network.

The Journal of Biological Chemistry
S M JonesK E Howell

Abstract

Current evidence suggests that phosphatidylinositol (PI) kinases and phosphatidylinositol transfer protein (PITP) are involved in driving vesicular traffic from yeast and mammalian trans-Golgi network (TGN). We have tested the interaction between these cytosolic proteins in an assay that measures the formation of constitutive transport vesicles from the TGN in a hepatocyte cell-free system. This reaction is dependent on a novel PI 3-kinase, and we now report that, under conditions of limiting cytosol, purified PI 3-kinase and PITP functionally cooperate to drive exocytic vesicle formation. This synergy was observed with both yeast and mammalian PITPs, and it also extended to the formation of PI 3-phosphate. These collective findings indicate that the PI 3-kinase and PITP synergize to form a pool of PI 3-phosphate that is essential for formation of exocytic vesicles from the hepatocyte TGN.

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J SalameroK E Howell
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·H B SkinnerV A Bankaitis
May 6, 1994·Cell·M Liscovitch, L C Cantley

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Citations

Sep 1, 2001·Cellular and Molecular Life Sciences : CMLS·F GuG Thomas
Apr 6, 2004·Biochemical and Biophysical Research Communications·Marie E MonacoPaul D Walden
Jul 19, 2002·Advances in Enzyme Regulation·Gursant S KularShamshad Cockcroft
Oct 18, 2002·Current Opinion in Cell Biology·Maria De MatteisDaniela Corda
May 14, 1999·Trends in Cell Biology·M G Roth
Apr 27, 2004·Nature Cell Biology·Anna GodiM Antonietta De Matteis
Jun 19, 2007·Nature Genetics·Christopher S PeacockMatthew Berriman
Jan 19, 2010·The Journal of Biological Chemistry·Brigitte HellerClark D Wells
Mar 22, 2002·Molecular Biology of the Cell·James G AlbVytas A Bankaitis
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·J P SimonD D Sabatini
Oct 20, 2006·Nature Chemical Biology·Kristina E IleVytas A Bankaitis
Sep 23, 1998·The Journal of Cell Biology·S S MolloyG Thomas
May 22, 2007·Biochimica Et Biophysica Acta·Carl J MousleyVytas A Bankaitis
Jun 5, 2007·Biochimica Et Biophysica Acta·Friedhelm SchroederAnn B Kier
Jan 17, 2015·Biochimica Et Biophysica Acta·Aby GrabonVytas A Bankaitis
Apr 9, 2001·Seminars in Cell & Developmental Biology·S Cockcroft
Oct 26, 2016·Chemistry and Physics of Lipids·Mark I McDermott, Carl J Mousley
Oct 26, 1999·The Journal of Biological Chemistry·Y FullwoodJ J Hsuan
Jul 23, 2004·Physiological Reviews·Michael G Roth
Oct 29, 2002·FEBS Letters·Victoria Allen-BaumeShamshad Cockcroft
Feb 4, 2005·American Journal of Physiology. Cell Physiology·Xiaorui ShiAlfred L Nuttall
Feb 4, 2006·Critical Reviews in Biochemistry and Molecular Biology·Scott E PhillipsEric A Gaucher
Aug 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Giuseppe ProcinoGiovanna Valenti

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