PMID: 9180160May 29, 1997Paper

A phospholipase D and protein kinase C inhibitor blocks the spreading of murine mammary adenocarcinoma cells altering f-actin and beta1-integrin point contact distribution

International Journal of Cancer. Journal International Du Cancer
J A Aguirre GhisoE Bal de Kier Joffé

Abstract

Spreading is a critical process involved in motility and growth of tumor cells during the metastatic cascade. Focal adhesion kinase, src-proteins and PKC have been reported to participate in the regulation of cytoskeleton organization in both normal and transformed cells during spreading. The role of other signaling enzymes such as PLD and PAP has not been studied during spreading in tumor cells. We now show that the spreading of murine mammary adenocarcinoma LM3 cells was significantly reduced by n-butanol, a PLD and PKC inhibitor, with a maximal inhibition of 54% (p < 0.001) in both the presence and absence of serum, as measured by phase-contrast microscopy. PMA only stimulated cell spreading over the control in the absence of serum and n-butanol inhibition was completely reversed by PMA treatment in both conditions. PA, the product of PLD activity, stimulated LM3 cell spreading and the same effect was observed with staurosporine. Spreading was enhanced when cells were seeded on collagen-IV- or fibronectin-coated surfaces and n-butanol could inhibit both integrin-derived signals. Cell spreading inhibition correlated with the absence of f-actin bundles and fewer beta1-integrin point contacts as determined by double immunofluor...Continue Reading

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Citations

Jan 22, 2000·The Biochemical Journal·M LiscovitchX Tang
Jan 13, 2009·Nature Chemical Biology·Sarah A ScottH Alex Brown
Feb 24, 2010·Molecular and Cellular Biology·Karen M HenkelsJulian Gomez-Cambronero
Nov 17, 2004·Molecular Carcinogenesis·Alejandro J UrtregerElisa D Bal de Kier Joffé
Jun 11, 2002·Biochemical and Biophysical Research Communications·Yingjie ShenDavid A Foster
Jul 31, 1998·Experimental Cell Research·V LadedaE Bal de Kier Joffé
Apr 27, 2004·Cancer Gene Therapy·Liliana M E FinocchiaroGerardo C Glikin
Apr 13, 2006·The Journal of Biological Chemistry·Jung Hwan KimSung Ho Ryu
Sep 24, 2014·Pharmacological Reviews·Ronald C BruntzH Alex Brown
Aug 12, 2003·Breast Cancer Research and Treatment·M G PetersE Bal de Kier Joffé
Jul 27, 1999·International Journal of Cancer. Journal International Du Cancer·A J UrtregerE Bal de Kier Joff

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