A phospholipidomic analysis of all defined human plasma lipoproteins.

Scientific Reports
Monireh DashtiFarhad Rezaee

Abstract

Since plasma lipoproteins contain both protein and phospholipid components, either may be involved in processes such as atherosclerosis. In this study the identification of plasma lipoprotein-associated phospholipids, which is essential for understanding these processes at the molecular level, are performed. LC-ESI/MS, LC-ESI-MS/MS and High Performance Thin Layer Chromatography (HPTLC) analysis of different lipoprotein fractions collected from pooled plasma revealed the presence of phosphatidylethanolamine (PE), phosphatidylinositol (PI), and sphingomyeline (SM) only on lipoproteins and phosphatidylcholine (PC), Lyso-PC on both lipoproteins and plasma lipoprotein free fraction (PLFF). Cardiolipin, phosphatidylglycerol (PG) and Phosphatidylserine (PS) were observed neither in the lipoprotein fractions nor in PLFF. All three approaches led to the same results regarding phospholipids occurrence in plasma lipoproteins and PLFF. A high abundancy of PE and SM was observed in VLDL and LDL fractions respectively. This study provides for the first time the knowledge about the phospholipid composition of all defined plasma lipoproteins.

References

Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·D Atkinson, D M Small
Nov 28, 1986·JAMA : the Journal of the American Medical Association·W P CastelliW B Kannel
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S ParthasarathyD Steinberg
Apr 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·J VaysseM Garnier
Oct 16, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·A SachinidisH Vetter
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·H DeguchiJ H Griffin
Dec 16, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·X C JiangA R Tall
Apr 9, 2001·Infection and Immunity·J H LevelsS J van Deventer
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Robert J Cushley, Mark Okon
Apr 18, 2003·Journal of Lipid Research·Jim W BurgessDaniel L Sparks
Nov 5, 2003·Journal of Lipid Research·Patricia G YanceyGeorge H Rothblat
Nov 17, 2004·Molecular and Cellular Biochemistry·C PiperiA Kalofoutis
Nov 18, 2004·Journal of Lipid Research·Axel SchlittXian-Cheng Jiang
Dec 4, 2004·Journal of Lipid Research·Jim W BurgessDaniel L Sparks
Jul 2, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ryo TaguchiTakao Shimizu
Jan 19, 2006·Proteomics·Farhad RezaeeJoost C M Meijers
Jan 31, 2006·Journal of Lipid Research·Ulf SommerCatherine E Costello
Feb 2, 2006·Journal of Lipid Research·Xianlin HanRichard W Gross
Sep 16, 2010·Journal of Proteome Research·Karla C S QueirozFarhad Rezaee

❮ Previous
Next ❯

Citations

Oct 5, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Michael R RaschBrian A Korgel
Jan 1, 2012·Scientifica·Maikel P Peppelenbosch
Jun 21, 2014·Mediators of Inflammation·Alok AgrawalAntonio E Rusiñol
Dec 15, 2015·European Journal of Nutrition·Aahana ShresthaAli A Moazzami
Dec 3, 2014·Biochimica Et Biophysica Acta·Sanja CurcicGunther Marsche
Feb 1, 2015·Archives of Toxicology·Cuicui GeZhifang Chai
Jan 21, 2015·Nanomedicine·Morteza MahmoudiHojatollah Vali
Dec 3, 2016·Scientific Reports·Miaoling LiFeng Wen
Oct 5, 2018·Antioxidants·Sotirios KiokiasVassilki Oreopoulou
Jun 13, 2015·F1000Research·Jan Voskuil
Jan 24, 2019·Lipids in Health and Disease·Sebastian RauschertBerthold Koletzko
Nov 1, 2018·Annual Review of Physiology·Bo Wang, Peter Tontonoz
Aug 30, 2019·Biomedical Chromatography : BMC·Rabia JaveedMuhammad Najam-Ul-Haq
Oct 24, 2019·Journal of Alzheimer's Disease : JAD·Matthew Wai Kin WongAnne Poljak
May 31, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nathalie NiyonzimaTerje Espevik
Jun 15, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Ville MännistöJussi Pihlajamäki
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·J E KyleT O Metz
Sep 29, 2014·Biomaterials Science·Mohammad J HajipourMorteza Mahmoudi
Sep 12, 2020·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Alessandro Di MinnoMatteo N D Di Minno
Apr 2, 2021·Frontiers in Endocrinology·Marco Di Gioia, Ivan Zanoni
Jul 1, 2021·The Journal of Nutritional Biochemistry·Marcos Yukio YoshinagaMarcelo Macedo Rogero
Aug 7, 2021·Metabolomics : Official Journal of the Metabolomic Society·Gakyung LeeByung Hwa Jung
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Youli WangJianmin Yuan

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.