PMID: 2489483Aug 22, 1989Paper

A photoactivable phospholipid analogue that specifically labels membrane cytoskeletal proteins of intact erythrocytes

Biochemistry
D PradhanR A Schlegel

Abstract

A radioactive photoactivable analogue of phosphatidylethanolamine, 2-(2-azido-4-nitro-benzoyl)-1-acyl-sn-glycero-3-phospho[14C]ethanolamine ([14C]AzPE), was synthesized. Upon incubation with erythrocytes in the dark, about 90% of [14C]AzPE spontaneously incorporated into the cells; of this fraction, about 90% associated with the membrane, all of it noncovalently. Upon photoactivation, 3-4% of the membrane-associated probe was incorporated into protein. Analysis of this fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as well as extraction of labeled membranes with alkali or detergent, showed that the probe preferentially labeled cytoskeletal proteins. [14C]AzPE appears to be a useful tool for the study of lipid-protein interactions at the cytoplasmic face of the plasma membrane of intact cells.

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J M TylerD Branton
Jan 1, 1979·Annual Review of Biochemistry·J A Op den Kamp
Jan 1, 1985·American Journal of Industrial Medicine·R BéginM Boctor
Nov 22, 1988·Biochimica Et Biophysica Acta·J BasuP Chakrabarti
Jan 1, 1988·The Journal of Clinical Investigation·A C RybickiR S Schwartz
Jan 22, 1988·Biochimica Et Biophysica Acta·K A ShifferS B Shohet
Jan 1, 1985·Annual Review of Cell Biology·V T Marchesi
Aug 17, 1973·Science·M S Bretscher
Mar 1, 1981·The Biochemical Journal·R Bisson, C Montecucco
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M Seigneuret, P F Devaux
Dec 31, 1980·Biochemical and Biophysical Research Communications·C M Cohen, C Korsgren

❮ Previous
Next ❯

Citations

Jan 1, 1994·Progress in Lipid Research·F Paltauf, A Hermetter
Apr 16, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M M ZegersD Hoekstra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.