A Photoactivatable α5 β1 -Specific Integrin Ligand

Chembiochem : a European Journal of Chemical Biology
Roshna V NairAránzazu del Campo

Abstract

The integrin α5 β1 is overexpressed in colon, breast, ovarian, lung and brain tumours, and has been identified as key component in mechanosensing. In order to study how dynamic changes in α5 β1 engagement affect cellular behaviour, photoactivatable derivatives of α5 β1 -specific ligands are presented in this article. A photoremovable protecting group (PRPG) was introduced into the ligand structure at a relevant position for integrin recognition. The presence of the chromophore temporarily inhibited ligand bioactivity. Light exposure at a cell-compatible dose efficiently cleaved the protecting group and restored functionality. The photoactive ligand had an azide end-functional group for covalent immobilisation onto biomaterials by click chemistry. Selective cell responses (attachment, spreading, migration) to the activated ligand on the surface are achieved by controlled exposure to light, at similar levels to the native ligand. Spatial and temporal control of the cellular response is demonstrated, including the possibility of in situ activation. Photoactivatable integrin-selective ligands in model microenvironments will allow the study of cellular behaviour in response to changes in the activation of individual integrins as con...Continue Reading

References

Feb 13, 2004·The Journal of Biological Chemistry·Akira KatsumiMartin Alexander Schwartz
Mar 19, 2008·Angewandte Chemie·Svea PetersenAranzazu del Campo
May 16, 2008·Chembiochem : a European Journal of Chemical Biology·Dominik HeckmannHorst Kessler
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Pere Roca-CusachsMichael P Sheetz
Nov 17, 2009·Journal of Cell Science·Adam ByronMartin J Humphries
Feb 2, 2010·HFSP Journal·Alexandre SpechtMaurice Goeldner
Feb 4, 2011·Chembiochem : a European Journal of Chemical Biology·Jesus M AizpuruaJosé Luis Castrillo
Nov 8, 2011·Chembiochem : a European Journal of Chemical Biology·Melanie WirknerAránzazu del Campo
Feb 12, 2013·FEBS Letters·Grace L LinChristopher S Chen
Mar 16, 2013·Bioorganic & Medicinal Chemistry·Sri Kamesh NarasimhanYan-Yeung Luk
Sep 7, 2013·Frontiers in Oncology·Udaya Kiran MarelliHorst Kessler
May 6, 2014·Nature Materials·Alberto Elosegui-ArtolaPere Roca-Cusachs
Nov 1, 2015·Experimental Cell Research·Keith Burridge, Christophe Guilluy
Jan 30, 2016·Nature Reviews. Drug Discovery·Klaus LeySanford Shattil
Feb 27, 2016·Organic & Biomolecular Chemistry·M Pilkington-MiksaL Manzoni
Jun 4, 2016·Angewandte Chemie·Carlos Mas-MorunoHorst Kessler
Jan 31, 2017·Journal of Medicinal Chemistry·Lisa M MillerAllan J B Watson
Nov 25, 2017·Nature Materials·Ning Wang

❮ Previous
Next ❯

Citations

Mar 25, 2019·Stem Cells International·Rosaria SantoroGiulio Pompilio
Jan 25, 2021·Advanced Drug Delivery Reviews·Teresa L Rapp, Cole A DeForest
Apr 24, 2019·Current Opinion in Colloid & Interface Science·Pradeep Bugga, Milan Mrksich
Jun 11, 2021·Advanced Healthcare Materials·Roshna V NairAránzazu Del Campo
Nov 6, 2018·ACS Applied Materials & Interfaces·Aleeza FarrukhAránzazu Del Campo
Nov 6, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Yijun ZhengAránzazu Del Campo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Related Papers

Methods in Molecular Biology
K von der MarkS L Goodman
Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists
M H GinsbergE F Plow
© 2022 Meta ULC. All rights reserved